首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的TGFB1含量。TGFB1（转化生长因子β1）是一种多功能细胞因子，几乎由所有细胞合成，具有调节细胞增殖、生长、分化和细胞运动等功能。其活性受潜伏相关肽（LAP）调节，通过结合细胞表面受体（TGFβRI和TGFβRII）激活下游信号通路，参与多种生理和病理过程，如发育、纤维化和癌症。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于基础科研领域，如探究疾病模型中TGF-β1的分泌动态、评估药物干预对细胞因子表达的影响，或分析免疫细胞与微环境间的信号调控机制，为炎症反应、肿瘤生物学及再生医学等研究方向提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P04202
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.2 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-97 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.6542.6362.6452.515 252.3182.4552.3872.257 12.51.7561.8861.8211.691 6.250.9971.0010.9990.869 3.120.5420.5120.5270.397 1.560.3010.3090.3050.175 0.780.2280.2380.2330.103 00.1290.1310.130 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse TGF-β1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled TGF-β1 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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