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产品描述：绵羊转化生长因子β1(TGFB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TGFB1含量。TGFB1是重要靶点。它是转化生长因子，在细胞生长、分化等多种生理活动中起关键作用。研究机制方面，其信号通路异常与肿瘤、纤维化等疾病密切相关，当前致力于通过药物干预该通路，以实现疾病的治疗和控制。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于绵羊免疫调控机制、炎症模型构建、生殖生理或疾病相关分子机制等科研领域，可用于探索TGF-β1在动物模型中的表达调控及生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P50414
种属：Ovis aries (Sheep)
样本类型：serum, plasma
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 86-102 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 82-99 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 93-110 | ||||||
回收率：
| The recovery of sheep TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 89-101 | |||||
| EDTA plasma (n=4) | 97 | 87-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
 ng/mlOD1OD2AverageCorrected10002.6952.6342.6652.5345002.1272.0982.1131.9822501.4291.4311.4301.2991250.7750.7980.7870.65662.50.4460.4540.4500.31931.250.3080.3250.3170.18615.60.2510.2620.2570.12600.1250.1360.131 | |||||||
货期：3-5 working days
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