首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转化生长因子β2(TGFβ2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFB2含量。TGFB2是一种重要靶点。它属于转化生长因子 - β超家族成员，在细胞生长、分化、凋亡及胚胎发育等多种生理过程中发挥关键作用。研究机制方面，其信号通路的异常激活与肿瘤、纤维化等多种疾病的发生发展密切相关，是药物研发的潜在方向。试剂盒检测范围为1.56 pg/mL-100 pg/mL，该产品特别适用于炎症反应研究、胚胎发育模型、纤维化疾病机制探索等科研场景，例如在肿瘤微环境分析中监测TGFβ2的表达动态，或在心血管研究中评估其参与组织重塑的调控作用。组织匀浆检测功能可支持局部病灶如脑、肝脏等器官的分子表达谱研究，为体外实验和动物模型提供可靠的定量检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
缩写：TGFB2
Uniprot No.：P27090
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 85-98 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 88-93 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 96-104 | |||||
回收率：
| The recovery of mouse TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 87-92 | ||||
| EDTA plasma (n=4) | 94 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.4322.5522.4922.352502.0412.0762.0591.919251.5461.6041.5751.43512.51.0021.0091.0060.8666.250.5090.5240.5170.3773.120.3340.3590.3470.2071.560.2380.2290.2340.09400.1350.1450.140 | ||||||
货期：3-5 working days
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