| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBL3 |
| Uniprot No | O95164 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-114aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSNVPADMINLRLILVSGKTKEFLFSPND SASDIAKHVYDNWPMDWEEEQVSSPNILRLIYQGRFLHGNVTLGALKLPF GKTTVMHLVARETLPEPNSQGQRNREKTGESNCC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBL3重组蛋白的3篇参考文献示例(注：以下内容为模拟文献，实际研究中请以真实文献为准)：
---
1. **文献名称**: *"Recombinant UBL3 Protein Facilitates Membrane Trafficking in Mammalian Cells"*
**作者**: Tanaka, K., et al.
**摘要**: 本研究成功表达并纯化了人源UBL3重组蛋白，探究其参与调控细胞内膜运输的分子机制。实验表明，UBL3通过与转运蛋白Sec16A相互作用，影响COPII囊泡的形成，进而调控分泌途径中的蛋白质运输。
---
2. **文献名称**: *"Structural Insights into UBL3-Mediated Protein Ubiquitination"*
**作者**: Chen, X., & Wang, L.
**摘要**: 通过X射线晶体学解析了重组UBL3蛋白的分子结构，发现其C端泛素样结构域与E3连接酶的相互作用界面。功能实验表明UBL3可作为适配分子，促进特定底物的泛素化修饰，为癌症相关通路研究提供新线索。
---
3. **文献名称**: *"UBL3 Recombinant Protein Modulates Autophagy via mTOR Signaling Pathway"*
**作者**: Park, S., et al.
**摘要**: 研究利用重组UBL3蛋白进行体外结合实验，发现其直接结合自噬关键蛋白LC3.并通过调控mTOR信号通路影响自噬活性。此发现为神经退行性疾病中UBL3的功能机制提供了实验依据。
---
如需查找真实文献，建议在 **PubMed** 或 **Google Scholar** 中搜索关键词：
`"UBL3 recombinant protein"`、`"UBL3 expression and purification"`、`"UBL3 functional analysis"`。
UBL3 (Ubiquitin-Like Protein 3) is a member of the ubiquitin-like protein family, which shares structural homology with ubiquitin but serves non-proteolytic regulatory roles. It is conserved across eukaryotes and is implicated in diverse cellular processes, including membrane trafficking, protein sorting, and signal transduction. UBL3 contains a characteristic ubiquitin-like (UBL) domain at its N-terminus, enabling interactions with other proteins, and a C-terminal region that may regulate its localization or function. Unlike ubiquitin, UBL3 is not directly involved in protein degradation but modulates pathways through post-translational modifications or protein-protein interactions.
Studies suggest UBL3 plays a role in regulating secretory pathways and extracellular vesicle (EV) cargo loading. It is proposed to act as a "sorting factor," directing specific proteins into EVs or guiding vesicle formation. UBL3 has also been linked to neural development, immune responses, and cancer progression, though its exact mechanisms remain under investigation. For example, UBL3 may influence receptor tyrosine kinase signaling or apoptosis regulation in tumor cells.
Recombinant UBL3 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains the native protein's structural and functional properties. Its production enables in vitro studies to dissect molecular interactions, enzymatic activities, and structural features. Researchers utilize recombinant UBL3 to explore its binding partners, post-translational modifications (e.g., phosphorylation), and role in EV-mediated intercellular communication. Additionally, it serves as a tool for developing diagnostic assays or therapeutic strategies targeting UBL3-associated pathways. Ongoing research aims to clarify UBL3's physiological relevance and potential as a biomarker or drug target in diseases like cancer or neurodegenerative disorders.
×
