| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBTD2 |
| Uniprot No | Q8WUN7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGGCVGA QHDSSGSLNE NSEGTGVALG RNQPLKKEKP KWKSDYPMTD GQLRSKRDEF WDTAPAFEGR KEIWDALKAA AHAFESNDHE LAQAIIDGAN ITLPHGALTE CYDELGNRYQ LPVYCLAPPI NMIEEKSDIE TLDIPEPPPN SGYECQLRLR LSTGKDLKLV VRSTDTVFHM KRRLHAAEGV EPGSQRWFFS GRPLTDKMKF EELKIPKDYV VQVIVSQPVQ NPTPVEN |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBTD2重组蛋白的3篇参考文献的简要总结(注：文献为虚构示例，实际研究中请核实真实来源)：
1. **"UBTD2 regulates proteasome activity through ubiquitin folding"**
*作者：Zhang Y, et al. (2020)*
**摘要**：研究利用重组UBTD2蛋白揭示了其通过调控泛素折叠过程增强蛋白酶体活性的分子机制，表明UBTD2在蛋白质降解通路中的关键作用。
2. **"Structural insights into UBTD2-ubiquitin interactions by recombinant protein analysis"**
*作者：Wang L, et al. (2018)*
**摘要**：通过重组UBTD2蛋白的晶体结构解析，发现其C端结构域特异性结合泛素分子，为UBTD2参与泛素信号传导的机制提供了结构基础。
3. **"UBTD2 overexpression promotes tumor growth via stabilizing EGFR in lung cancer"**
*作者：Chen X, et al. (2021)*
**摘要**：利用重组UBTD2蛋白进行体外实验，证实其通过稳定EGFR蛋白促进肺癌细胞增殖，提示UBTD2作为癌症治疗潜在靶点。
**提示**：实际文献可能较少，建议通过PubMed或Google Scholar以“UBTD2 recombinant protein”“UBTD2 ubiquitination”等关键词检索最新研究。若需具体文献协助，可提供更多背景信息。
**Background of UBTD2 Recombinant Protein**
UBTD2 (Ubiquitin Domain-Containing Protein 2) is a conserved eukaryotic protein implicated in regulating ubiquitin-dependent cellular processes. As a member of the ubiquitin-associated (UBA) domain-containing protein family, UBTD2 interacts with components of the ubiquitin-proteasome system (UPS), which governs protein degradation, quality control, and signaling. Structurally, UBTD2 contains a central UBA domain that facilitates binding to ubiquitin or ubiquitinated substrates, alongside conserved C-terminal motifs that mediate protein-protein interactions.
Studies suggest UBTD2 plays dual roles in cellular homeostasis. It acts as a scaffold to modulate ubiquitin ligase activity, influencing substrate specificity or stability, and may regulate cell cycle progression, DNA repair, or stress responses. Dysregulation of UBTD2 has been linked to pathologies, including cancer, where its overexpression or depletion correlates with tumor growth, metastasis, or chemoresistance.
Recombinant UBTD2 protein is engineered via heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for mechanistic studies. It enables *in vitro* assays to decipher UBTD2’s binding partners, enzymatic interactions, or structural features. Additionally, recombinant UBTD2 serves as an antigen for antibody development or a tool to screen therapeutic inhibitors targeting UPS-related diseases. Current research focuses on elucidating its precise molecular functions and therapeutic potential, positioning UBTD2 as a promising candidate for understanding ubiquitin signaling and drug discovery.
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