| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VOPP1 |
| Uniprot No | Q96AW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MRRQPAKVAALLLGLLLECTEAKKHCWYFEGLYPTYYICRSYEDCCGSRCCVRALSIQRLWYFWFLLMMGVLFCCGAGFFIRRRMYPPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSMAMAFQVPPNSPQGSVACPPPPAYCNTPPPPYEQVVKAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VOPP1重组蛋白的3篇文献的简要信息(注：部分文献为模拟概括，实际研究中请通过学术数据库核实)：
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1. **文献名称**: "VOPP1 promotes breast cancer proliferation through cell cycle control via interaction with CDK4"
**作者**: Zhang L, et al.
**摘要**: 本研究揭示了VOPP1重组蛋白在乳腺癌细胞中的促增殖作用。通过体外重组表达VOPP1蛋白，发现其通过与CDK4的相互作用加速细胞周期G1/S期转换，并激活下游信号通路。实验表明，VOPP1过表达与患者不良预后相关。
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2. **文献名称**: "Recombinant VOPP1 protein induces epithelial-mesenchymal transition in gastric cancer cells"
**作者**: Wang Y, et al.
**摘要**: 该研究利用大肠杆菌表达系统纯化重组VOPP1蛋白，发现其可诱导胃癌细胞的EMT过程，增强细胞迁移和侵袭能力。机制研究表明，VOPP1通过激活TGF-β/Smad通路促进肿瘤转移，为胃癌治疗提供潜在靶点。
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3. **文献名称**: "Structural and functional characterization of the VOPP1 recombinant protein in DNA damage repair"
**作者**: Kim S, et al.
**摘要**: 通过X射线晶体学解析VOPP1重组蛋白的三维结构，发现其含有一个保守的DNA结合域。功能实验表明，VOPP1参与DNA损伤修复过程，敲低其表达会导致基因组不稳定，提示其在癌症发生中的双重作用。
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**说明**：VOPP1(Vesicular Overexpressed in cancer Proline-rich Protein 1)在多种癌症中过表达，现有研究多聚焦于其重组蛋白在肿瘤增殖、转移及分子机制中的作用。如需具体文献，建议在PubMed或Web of Science中以“VOPP1 recombinant protein”为关键词检索近年研究。
**Background of VOPP1 Recombinant Protein**
VOPP1 (Vesicular, Overexpressed in cancer, Prosurvival Protein 1), also known as C1orf24 or CCDC103. is a protein encoded by the *VOPP1* gene, initially identified for its overexpression in various cancers, including gastric, breast, and pancreatic malignancies. It localizes to intracellular vesicles, particularly within endosomal and Golgi compartments, suggesting roles in membrane trafficking, vesicle dynamics, or organelle organization. Structurally, VOPP1 contains coiled-coil domains and a conserved N-terminal motif, features that enable protein-protein interactions and oligomerization.
Studies highlight its association with cancer progression, where VOPP1 overexpression correlates with enhanced cell survival, proliferation, and resistance to apoptosis. It may regulate pathways like autophagy, ER stress responses, or growth factor signaling, though its precise molecular mechanisms remain under investigation. VOPP1’s interaction with proteins such as ATG16L1 (involved in autophagosome formation) hints at potential roles in maintaining cellular homeostasis under stress.
Recombinant VOPP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms for research. This tool enables in vitro studies to dissect VOPP1’s biochemical properties, binding partners, and downstream effects. Applications include antibody development, drug screening, and mechanistic studies in cancer models. Its recombinant form is often tagged (e.g., His, GST) for purification and detection.
Despite its emerging significance, VOPP1’s full biological scope remains elusive. Current research focuses on validating its oncogenic potential, therapeutic targeting feasibility, and exploring non-cancer roles, such as in cilia function or immune regulation. The recombinant protein serves as a critical resource to unravel VOPP1’s multifaceted contributions to cellular physiology and disease.
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