| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS26A |
| Uniprot No | O75436 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSFLGGFFGPICEIDIVLNDGETRKMA EMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIE LFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVR LRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIE FEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTET ETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLV LVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VPS26A重组蛋白的3篇关键文献信息(示例基于公开研究整理，非真实文献)：
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1. **文献名称**: *Structural insights into the retromer complex through VPS26A recombinant protein analysis*
**作者**: Collins et al. (2018)
**摘要**: 通过重组表达人源VPS26A蛋白并解析其晶体结构，揭示了其与VPS35蛋白的相互作用界面，阐明retromer复合体在囊泡运输中的构象变化机制。
2. **文献名称**: *VPS26A recombinant protein rescues endosomal sorting defects in cellular models*
**作者**: Zhang et al. (2020)
**摘要**: 研究利用重组VPS26A蛋白在体外细胞模型中恢复retromer功能缺失导致的溶酶体酶分选异常，证明其在神经退行性疾病治疗中的潜在应用价值。
3. **文献名称**: *Biochemical characterization of recombinant VPS26A reveals ATPase-independent cargo recognition*
**作者**: Mukherjee & Bonifacino (2016)
**摘要**: 通过重组蛋白互作实验，发现VPS26A不依赖ATP水解即可识别特定货物蛋白(如CI-MPR)，为retromer复合体的分选机制提供了新见解。
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(注：以上文献为示例，实际引用需查询真实数据库如PubMed/Google Scholar，建议结合关键词“VPS26A recombinant protein”或“VPS26A retromer structure”筛选近年高引论文。)
VPS26A (Vacuolar Protein Sorting 26 Homolog A) is a critical component of the retromer complex, a conserved protein assembly involved in intracellular trafficking and endosomal sorting. This 38-kDa protein plays a pivotal role in maintaining cellular homeostasis by facilitating the retrieval of transmembrane cargos from endosomes to the trans-Golgi network or plasma membrane. The retromer complex, composed of VPS26 (with isoforms VPS26A and VPS26B), VPS29. and VPS35. coordinates cargo recognition and membrane deformation through interactions with sorting nexins. VPS26A specifically contributes to cargo recognition by binding to sorting signals on target proteins, including Wntless and GLUT1. thus regulating processes like Wnt signaling and glucose metabolism.
Recombinant VPS26A protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for structural and functional studies. Its recombinant form enables researchers to investigate retromer-mediated trafficking mechanisms, dissect molecular interactions, and model diseases linked to VPS26A dysfunction, such as neurodegenerative disorders. Mutations in VPS26A have been associated with altered retromer stability and impaired cargo sorting, potentially contributing to Alzheimer's disease pathology through dysregulated APP processing. Additionally, recombinant VPS26A serves as a tool for drug discovery, allowing screening of compounds targeting retromer-associated pathways. Recent studies also explore its role in cancer metastasis and autophagy regulation, highlighting its broad biological significance. The availability of recombinant VPS26A accelerates mechanistic insights into cellular trafficking and therapeutic development for related diseases.
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