首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人乙醛脱氢酶(ALDH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ALDH2含量。ALDH2 是一种重要的醛脱氢酶。它在人体代谢乙醛等醛类物质中发挥关键作用。研究表明，其基因多态性会影响酶活性，进而与酒精代谢、心血管疾病等相关。解析其作用机制有助于了解疾病发生发展过程，为相关疾病防治提供新思路。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于酒精性肝损伤模型研究、肿瘤耐药机制探索、干细胞功能分析等科研领域，支持离体组织（如肝脏、脑组织）的酶活性关联性研究，为体外水平研究ALDH表达调控提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acetaldehyde dehydrogenase 2 ELISA Kit; Aldehyde dehydrogenase 2 family (mitochondrial) ELISA Kit; Aldehyde dehydrogenase 2 family ELISA Kit; Aldehyde dehydrogenase mitochondrial ELISA Kit; Aldehyde dehydrogenase; mitochondrial ELISA Kit; ALDH 2 ELISA Kit; ALDH class 2 ELISA Kit; ALDH E2 ELISA Kit; ALDH-E2 ELISA Kit; Aldh2 ELISA Kit; ALDH2_HUMAN ELISA Kit; ALDHI ELISA Kit; ALDM ELISA Kit; Liver mitochondrial ALDH ELISA Kit; MGC1806 ELISA Kit; Mitochondrial aldehyde dehydrogenase 2 ELISA Kit; MS767 ELISA Kit; Nucleus encoded mitochondrial aldehyde dehydrogenase 2 ELISA Kit
缩写：ALDH2
Uniprot No.：P05091
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-106 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.3152.4422.3792.20725001.6641.7851.7251.55312501.1341.2241.1791.0076250.7460.7640.7550.5833120.5270.5120.5200.3481560.3510.3640.3580.186780.2520.2590.2560.08400.1710.1720.172 | ||||||
货期：3-5 working days
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