| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VTN |
| Uniprot No | P04004 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-478aa |
| 氨基酸序列 | DQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL |
| 预测分子量 | 52.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VTN(Vitronectin)重组蛋白研究的虚构参考文献示例(仅供格式参考，非真实文献)：
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1. **标题**: *Efficient production of recombinant human VTN in Escherichia coli for extracellular matrix applications*
**作者**: Zhang L, et al.
**摘要**: 研究报道了一种利用大肠杆菌表达系统高效生产重组VTN蛋白的方法，通过优化密码子和纯化工艺，获得高纯度蛋白，并验证其在细胞粘附和神经干细胞培养中的应用潜力。
2. **标题**: *Functional analysis of VTN recombinant protein in modulating angiogenesis and tumor metastasis*
**作者**: Gupta S, Patel RK.
**摘要**: 探讨重组VTN蛋白通过整合素信号通路调控血管生成的作用，发现其通过αvβ3受体抑制肿瘤细胞迁移，为癌症治疗提供新靶点。
3. **标题**: *Structural and stability characterization of a novel VTN variant for 3D bioprinting scaffolds*
**作者**: Müller J, et al.
**摘要**: 开发了一种热稳定性增强的重组VTN变体，结合3D生物打印技术构建仿生支架，显著提升干细胞的存活率和分化效率。
4. **标题**: *Cost-effective purification of recombinant VTN using affinity tag-free strategies*
**作者**: Kim H, Lee SM.
**摘要**: 提出一种无标签的重组VTN纯化方案，通过离子交换层析和疏水相互作用层析联用，降低生产成本，同时保持蛋白生物活性。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。如需具体文献推荐，建议补充研究关键词(如疾病方向或技术方法)。
Vitronectin (VTN) is a multifunctional extracellular matrix (ECM) glycoprotein involved in cell adhesion, migration, and tissue remodeling. It plays critical roles in regulating coagulation, complement activation, and cell signaling by interacting with integrins, heparin, plasminogen activator inhibitors, and other ECM components. Native VTN exists in two forms: a single-chain monomer in the ECM and a processed two-chain form in blood plasma. Its structure includes an Arg-Gly-Asp (RGD) motif for integrin binding, a heparin-binding domain, and a hemopexin-like domain, enabling diverse biological interactions.
Recombinant VTN proteins are engineered using DNA technology to mimic these functional properties, often expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation. This avoids batch variability and ethical concerns associated with animal-derived VTN. Recombinant VTN is indispensable in stem cell research, particularly for maintaining pluripotency in human embryonic and induced pluripotent stem cells (hESCs/iPSCs) under defined, xeno-free culture conditions. It also serves as a coating substrate for primary cell cultures, enhancing attachment and survival in vitro.
In regenerative medicine, VTN-functionalized scaffolds promote tissue repair by guiding cell behavior. Its role in pathogen entry—such as mediating hepatitis C virus (HCV) attachment via heparin-binding domains—has spurred interest in antiviral therapeutic strategies. Additionally, recombinant VTN’s standardized production ensures reproducibility in drug screening and disease modeling, aligning with Good Manufacturing Practice (GMP) requirements for clinical applications. By combining biological fidelity with scalable manufacturing, recombinant VTN bridges translational research and therapeutic development, offering a versatile tool for advancing cell-based therapies and precision medicine.
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