| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL10RB |
| Uniprot No | Q08334 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-220aa |
| 氨基酸序列 | MVPPPENVRMNSVNFKNILQWESPAFAKGNLTFTAQYLSYRIFQDKCMNT TLTECDFSSLSKYGDHTLRVRAEFADEHSDWVNITFCPVDDTIIGPPGMQ VEVLADSLHMRFLAPKIENEYETWTMKNVYNSWTYNVQYWKNGTDEKFQI TPQYDFEVLRNLEPWTTYCVQVRGFLPDRNKAGEWSEPVCEQTTHDETVP SVDHHHHHH |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL10RB重组蛋白的参考文献示例(内容基于合理推测，非真实文献)：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant Human IL10RB Protein*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人IL10RB蛋白，通过X射线晶体学解析其三维结构，揭示了其与IL10的结合界面，为靶向IL10信号通路的药物设计提供了结构基础。
2. **文献名称**：*IL10RB Recombinant Protein Modulates Inflammatory Responses in Murine Macrophages*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞系表达的重组IL10RB蛋白，证明其通过增强IL10介导的抗炎信号抑制巨噬细胞中促炎因子(如TNF-α、IL-6)的分泌，提示其潜在治疗慢性炎症疾病的应用价值。
3. **文献名称**：*Dysregulation of IL10RB in Colorectal Cancer and Rescue by Recombinant Protein Delivery*
**作者**：Wang Y, et al.
**摘要**：研究发现结直肠癌组织中IL10RB表达下调，体外实验表明递送重组IL10RB蛋白可恢复IL10信号通路活性，抑制肿瘤细胞增殖并促进凋亡，为癌症免疫治疗提供了新策略。
4. **文献名称**：*Interactions Between IL10RB and IL22RA1 Recombinant Proteins in Epithelial Immunity*
**作者**：Garcia-Ruiz C, et al.
**摘要**：通过表面等离子共振技术(SPR)分析重组IL10RB与IL22RA1的相互作用，发现两者协同调控肠道上皮细胞屏障功能，为黏膜免疫疾病的机制研究提供了实验依据。
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注：以上文献为示例性内容，实际引用时建议通过PubMed或Web of Science检索真实文献。
Interleukin-10 receptor subunit beta (IL10RB), a key component of the interleukin-10 receptor complex, plays a critical role in mediating anti-inflammatory and immunomodulatory signals. It belongs to the class II cytokine receptor family and forms a heterodimeric receptor with IL10RA (alpha subunit) to bind IL10. a cytokine essential for limiting excessive immune responses and maintaining tissue homeostasis. Beyond IL10 signaling, IL10RB also pairs with other receptor subunits to mediate signaling for IL-22. IL-26. and interferon-λ (IFN-λ), highlighting its functional versatility in regulating both innate and adaptive immunity.
Structurally, IL10RB is a transmembrane protein composed of an extracellular domain with conserved fibronectin type III motifs, a single transmembrane helix, and a cytoplasmic domain involved in signal transduction. Its expression is widespread across immune cells (e.g., macrophages, T cells) and epithelial tissues, underscoring its role in mucosal immunity and barrier protection. Recombinant IL10RB protein is typically produced in mammalian or insect expression systems to ensure proper post-translational modifications, often fused with tags (e.g., Fc, His-tag) for purification and functional studies.
Research on recombinant IL10RB has advanced understanding of receptor-ligand interactions, downstream JAK-STAT signaling pathways, and its dysregulation in diseases. Mutations in the IL10RB gene are linked to severe early-onset inflammatory bowel disease (IBD), emphasizing its clinical relevance. Therapeutic strategies targeting IL10RB, including recombinant proteins or gene therapy, are being explored to restore IL10 signaling in IBD patients. Additionally, IL10RB's involvement in cancer immunotherapy and viral infection responses (via IFN-λ) makes it a multifaceted target for immune-related therapies. Its recombinant form remains a vital tool for mechanistic studies and drug development.
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