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产品描述：猪补体蛋白3(C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3含量。C3作为靶点颇具研究价值。它是补体系统的关键成分，在免疫防御等多种生理过程中发挥核心作用。其研究机制主要聚焦于补体激活途径及下游效应，旨在通过调控C3功能，开发针对自身免疫病、炎症等疾病的治疗新策略。试剂盒检测范围为0.625 μg/mL-10 μg/mL，在科研领域，本产品可用于探究猪免疫相关疾病（如细菌/病毒感染、自身免疫性疾病）模型中补体系统的激活机制，评估疫苗或药物干预对补体通路的影响，亦可应用于比较不同遗传背景或饲养条件下猪群体C3表达水平的差异。组织匀浆检测功能还可支持局部炎症或器官特异性免疫反应的研究，例如肠道、肝脏等组织中补体活化与病理进程的关联分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C3 ELISA Kit; Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA Kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA Kit; ASP ELISA Kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA Kit
缩写：C3
Uniprot No.：P01025
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μg/mL-10 μg/mL
灵敏度：0.625 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 84-103 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 83-102 | |||||
回收率：
| The recovery of pig C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 88-108 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average100.0830.0840.08450.1850.1760.1812.50.3490.3520.3511.250.7690.7760.7730.6251.2851.2921.28902.1402.1512.146 | ||||||
货期：3-5 working days
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