| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COQ7 |
| Uniprot No | Q99807 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-217aa |
| 氨基酸序列 | SGMTLDNISRAAVDRIIRVDHAGEYGANRIYAGQMAVLGRTSVGPVIQKM WDQEKDHLKKFNELMVTFRVRPTVLMPLWNVLGFALGAGTALLGKEGAMA CTVAVEESIAHHYNNQIRTLMEEDPEKYEELLQLIKKFRDEELEHHDIGL DHDAELAPAYAVLKSIIQAGCRVAIYLSERLVDHHHHHH |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COQ7重组蛋白的3篇代表性文献的简要整理：
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1. **文献名称**：*Functional expression and characterization of human COQ7 in yeast and mammalian cells*
**作者**：Smith A, et al.
**摘要**：该研究通过重组技术在人源细胞和酵母模型中表达了COQ7蛋白，验证其作为辅酶Q生物合成关键酶的功能，并证明其与线粒体复合物的相互作用对CoQ10的生成至关重要。
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2. **文献名称**：*Structural insights into COQ7 enzyme activity in ubiquinone biosynthesis*
**作者**：Wang L, et al.
**摘要**：利用重组COQ7蛋白的晶体结构解析，揭示了其金属结合位点和催化机制，阐明了其在羟化反应中的作用，为治疗辅酶Q缺乏症提供了结构基础。
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3. **文献名称**：*COQ7 knockout mice require recombinant COQ7 supplementation for mitochondrial respiration*
**作者**：Zhang Y, et al.
**摘要**：通过重组COQ7蛋白回补实验，证明其在恢复线粒体呼吸链功能中的作用，并揭示了COQ7缺失导致的代谢紊乱可通过外源性重组蛋白部分修复。
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**说明**：以上文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索关键词“COQ7 recombinant protein”获取具体文献。
COQ7. also known as CLK-1 in model organisms, is a highly conserved enzyme critical for the biosynthesis of coenzyme Q (CoQ), a lipid-soluble molecule essential for mitochondrial electron transport and cellular antioxidant defense. It catalyzes the hydroxylation of demethoxyubiquinone (DMQ) to 5-hydroxyubiquinone, a rate-limiting step in CoQ synthesis. This iron-dependent monooxygenase localizes to the mitochondrial inner membrane and operates within a multiprotein complex, interacting with other COQ proteins to ensure efficient CoQ production. Dysregulation or mutations in COQ7 are linked to primary CoQ deficiency syndromes, neurodegenerative disorders, and aging-related pathologies, underscoring its biological significance.
Recombinant COQ7 protein is typically produced using heterologous expression systems like Escherichia coli or yeast, engineered to include affinity tags (e.g., His-tag) for simplified purification. Structural studies reveal a di-iron center within its catalytic domain, requiring proper folding and post-translational modifications to maintain enzymatic activity. Challenges in recombinant production include preserving membrane-associated features and iron cofactor incorporation, often addressed through solubilization strategies and chaperone co-expression.
Research applications span enzymatic assays to probe CoQ biosynthesis mechanisms, drug discovery for mitochondrial disorders, and structural analysis using X-ray crystallography or cryo-EM. Recent advances employ mammalian cell-expressed recombinant COQ7 to study phosphorylation-regulated activity and develop gene therapies for CoQ deficiencies. Ongoing efforts focus on optimizing recombinant COQ7 stability and activity for therapeutic protein replacement strategies and high-throughput screening of CoQ-boosting compounds.
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