Recombinant Human CTRB1 protein

中文名： 糜蛋白酶原B1(CTRB1)重组蛋白
别名： CTRB1；CTRB；Chymotrypsinogen B

货号: PA1000-3750

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	CTRB1
Uniprot No	P17538
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	19-263aa
氨基酸序列	CGVPAIHPVLSGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISED WVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVN NDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANK TPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLV CQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAANVDHHH HHH
预测分子量	27 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CTRB1重组蛋白的3篇参考文献示例(注：部分内容基于真实研究方向调整，具体文献需根据实际检索确认)：

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1. **标题**：*"A Chymotrypsinogen (CTRB1) Gene Variant Affects Pancreatitis Risk and Protein Stability"*

**作者**：Rosendahl J, et al.

**摘要**：该研究探讨了CTRB1基因变异(如p.G214R)与慢性胰腺炎易感性的关联，并利用重组CTRB1蛋白实验证明该突变导致酶原稳定性下降，影响胰蛋白酶活性调控。

2. **标题**：*"Expression and Purification of Recombinant Human CTRB1 in Pichia pastoris"*

**作者**：Zhang Y, et al.

**摘要**：文章描述了在毕赤酵母系统中高效表达重组人CTRB1蛋白的优化方法，通过亲和层析纯化获得高活性蛋白，并验证其酶动力学特性，为功能研究提供可靠工具。

3. **标题**：*"Structural Insights into CTRB1 Function: Implications for Pancreatic Disease Mechanisms"*

**作者**：Whitcomb DC, et al.

**摘要**：通过X射线晶体学解析重组CTRB1的三维结构，揭示其底物结合位点及关键催化残基，结合功能实验阐明其与胰蛋白酶原自身激活异常相关的分子机制。

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**说明**：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台以关键词“CTRB1 recombinant protein”“Chymotrypsin B1 expression”检索近期论文，并筛选涉及重组表达、功能机制或疾病关联的研究。

背景信息

**Background of CTRB1 Recombinant Protein**

Chymotrypsin B1 (CTRB1) is a serine protease encoded by the *CTRB1* gene, primarily synthesized in the pancreas as an inactive zymogen (pro-CTRB1). It is secreted into the small intestine, where it is activated by trypsin-mediated cleavage to participate in dietary protein digestion. CTRB1 specifically hydrolyzes peptide bonds at aromatic residues (e.g., tyrosine, phenylalanine), playing a critical role in nutrient absorption.

The recombinant CTRB1 protein is produced via molecular cloning, typically expressed in heterologous systems like *E. coli* or mammalian cells, followed by purification to ensure high enzymatic activity and purity. This engineered form retains the functional properties of native CTRB1. enabling studies on its structure, substrate specificity, and regulatory mechanisms.

Research on recombinant CTRB1 has expanded its applications beyond basic enzymology. It serves as a tool to investigate pancreatic disorders, such as chronic pancreatitis, where altered CTRB1 activity or genetic variants (e.g., *CTRB1* polymorphisms) are linked to disease susceptibility. Additionally, CTRB1 interacts with pancreatic secretory trypsin inhibitor (SPINK1), and their imbalance may contribute to pathological protease activation.

In biomedicine, recombinant CTRB1 aids in drug discovery, particularly for designing protease inhibitors or enzyme replacement therapies. It also has diagnostic potential, as CTRB1 levels in serum or feces may reflect pancreatic function. Furthermore, studies explore its role in cancer, where aberrant protease activity influences tumor progression.

Overall, CTRB1 recombinant protein is a vital resource for understanding pancreatic biology, disease mechanisms, and therapeutic development, bridging gaps between biochemical research and clinical applications.