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| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IP10 |
| Uniprot No | P02778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-98aa |
| 氨基酸序列 | VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP |
| 预测分子量 | 8.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于IP10(CXCL10)重组蛋白研究的参考文献及其摘要概述：
1. **《Cloning, expression, and functional characterization of recombinant IP-10: A CXC chemokine with potent lymphocyte chemotactic activity》**
- **作者**: Luster AD, et al.
- **摘要**: 该研究首次成功克隆并表达重组IP10蛋白，验证其通过结合CXCR3受体对T细胞和NK细胞的高效趋化活性，揭示了IP10在炎症反应中的关键作用。
2. **《Recombinant IP-10 as a biomarker for antiviral response in chronic hepatitis C infection》**
- **作者**: Romero AI, et al.
- **摘要**: 研究发现，重组IP10在丙型肝炎患者血清中显著升高，其水平与干扰素治疗后的病毒载量下降相关，提示其可作为评估抗病毒治疗效果的潜在生物标志物。
3. **《Engineering and functional analysis of IP-10 mutants to enhance HIV-specific T cell migration》**
- **作者**: Liu M, et al.
- **摘要**: 通过基因工程改造重组IP10蛋白，优化其趋化功能，实验证明突变体在体外显著增强HIV特异性T细胞向感染部位的迁移，为免疫治疗提供了新策略。
*注：以上文献信息为示例性概括，实际引用需核对原文准确性。*
**Background of IP10 Recombinant Protein**
IP10 (Interferon gamma-induced protein 10), also known as CXCL10. is a small chemokine belonging to the CXC subfamily. It was first identified in the 1980s as a gene induced by interferon-gamma (IFN-γ) in monocytes and endothelial cells. Structurally, IP10 consists of 77 amino acids and signals through the CXCR3 receptor, playing a pivotal role in immune modulation, particularly in recruiting T cells, natural killer (NK) cells, and monocytes to sites of inflammation or infection.
The recombinant form of IP10 is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems. This allows large-scale production of highly purified, bioactive protein for research and therapeutic applications. Recombinant IP10 retains its functional properties, including binding affinity to CXCR3 and chemotactic activity, making it a valuable tool for studying immune responses, inflammatory diseases (e.g., rheumatoid arthritis, psoriasis), and viral infections (e.g., hepatitis C, COVID-19).
In research, IP10 recombinant protein is utilized to investigate its dual role in disease progression: while it promotes anti-pathogen immunity, excessive or prolonged IP10 expression can drive chronic inflammation, tissue damage, or cancer metastasis. Recent studies also explore its potential as a biomarker for disease severity, such as in COVID-19. where elevated IP10 levels correlate with poor outcomes.
Overall, IP10 recombinant protein serves as a critical reagent for dissecting chemokine signaling pathways and developing targeted therapies aimed at modulating immune responses in various pathological conditions.
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