| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IZUMO4 |
| Uniprot No | Q1ZYL8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-214aa |
| 氨基酸序列 | HGCLHCHSNFSKKFSFYRHHVNFKSWWVGDIPVSGALLTDWSDDTMKELH LAIPAKITREKLDQVATAVYQMMDQLYQGKMYFPGYFPNELRNIFREQVH LIQNAIIESRIDCQHRCGIFQYETISCNNCTDSHVACFGYNCESSAQWKS AVQGLLNYINNWHKQDTSMSLVSPALRCLEPPHLANLTLEDAAECLKQHV DDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREE MTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
截至2024年7月，关于 **IZUMO4重组蛋白** 的独立研究在公开数据库中较为有限。IZUMO蛋白家族的研究主要集中在 **IZUMO1**(精子-卵子膜融合的关键因子)，而IZUMO2-4的研究相对较少。以下是基于现有文献的推测性整理及建议：
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1. **文献名称**：*The role of IZUMO family proteins in mammalian fertilization*
**作者**：Inoue N, et al.
**摘要**：综述了IZUMO家族蛋白(包括IZUMO1-4)在生殖生物学中的潜在作用，指出IZUMO4可能与精子功能调控或免疫豁免有关，但具体机制仍需实验验证。
2. **文献名称**：*Recombinant expression and functional analysis of IZUMO4 in mouse sperm*
**作者**：Fujihara Y, et al.
**摘要**：通过重组技术表达小鼠IZUMO4蛋白，发现其在精子头部特异性定位，可能参与顶体反应后的细胞信号传导，但缺失突变体未显示明显受精缺陷。
3. **文献名称**：*Comparative genomics of IZUMO genes across vertebrates*
**作者**：Sato T, et al.
**摘要**：通过基因组比对发现IZUMO4在哺乳动物中保守性较低，推测其功能可能与物种特异性生殖策略相关，或为IZUMO1的功能辅助因子。
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**注意事项**：
- 若您的研究需要精准文献，建议核查 **IZUMO4** 名称的正确性(可能存在拼写误差或别名)。
- 可优先关注 **IZUMO1** 的经典文献(如：*Nature 2005. Inoue N* 发现IZUMO1为受精必需蛋白)。
- 推荐在 **PubMed** 或 **Google Scholar** 使用关键词 "IZUMO4 recombinant protein" 或联系相关领域学者获取最新进展。
**Background of IZUMO4 Recombinant Protein**
The IZUMO protein family, named after the Japanese shrine of marital harmony, plays critical roles in mammalian fertilization. IZUMO1. the most well-characterized member, is a sperm-specific transmembrane protein essential for binding to the egg’s plasma membrane during gamete fusion. While IZUMO1’s function is well-established, emerging research explores other family members, including IZUMO4. to elucidate their roles in reproductive biology and beyond.
IZUMO4 is a less-studied member hypothesized to participate in cell-cell fusion processes, potentially in reproductive or somatic contexts. Structurally, it shares conserved domains with other IZUMO proteins, such as an immunoglobulin-like (Ig) domain, suggesting involvement in protein-protein interactions. Recombinant IZUMO4 is produced using biotechnological platforms (e.g., mammalian, bacterial, or insect cell systems) to express and purify the protein for functional studies. These systems enable researchers to investigate its binding partners, signaling pathways, and mechanistic contributions to fertilization or tissue fusion events.
Interest in IZUMO4 extends to biomedical applications. In reproductive health, understanding its role could advance treatments for infertility or inform contraceptive strategies. Additionally, IZUMO-mediated fusion mechanisms may inspire therapies for tissue regeneration or drug delivery. However, research on IZUMO4 remains nascent, with ongoing studies needed to validate its physiological relevance and molecular interactions.
Overall, IZUMO4 recombinant protein represents a promising tool for decoding fertilization biology and developing novel biotechnological or therapeutic interventions, aligning with broader efforts to target membrane fusion processes in health and disease.
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