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| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCL11 |
| Uniprot No | P51671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-97aa |
| 氨基酸序列 | GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDICADPKKKWVQD SMKYLDQKSP TPKP |
| 预测分子量 | 8.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCL11重组蛋白的示例参考文献(内容为虚构，供参考)：
1. **标题**：重组人CCL11蛋白在大肠杆菌中的高效表达与纯化
**作者**：Smith J, et al.
**摘要**：本研究通过构建pET载体在大肠杆菌中高效表达重组人CCL11蛋白，采用镍柱亲和层析纯化，获得高纯度(>95%)蛋白。功能实验证实其可诱导嗜酸性粒细胞迁移，为后续疾病模型研究提供材料。
2. **标题**：CCL11重组蛋白在哮喘小鼠模型中的作用机制
**作者**：Lee H, et al.
**摘要**：通过鼻内递送重组CCL11蛋白至哮喘模型小鼠，发现其显著增强肺部嗜酸性粒细胞浸润及炎症反应，证实CCL11通过CCR3受体介导过敏性气道疾病的病理过程。
3. **标题**：哺乳动物细胞表达系统制备功能性CCL11及其结构分析
**作者**：Garcia R, et al.
**摘要**：利用HEK293细胞表达重组CCL11.结合质谱和圆二色光谱分析其正确折叠及二硫键形成。研究揭示了CCL11的构效关系，为靶向药物设计提供结构基础。
4. **标题**：CCL11与阿尔茨海默病的相关性：重组蛋白的体外神经毒性研究
**作者**：Wang Y, et al.
**摘要**：体外实验表明，重组CCL11可激活小胶质细胞并促进神经炎症因子释放，提示其可能通过血脑屏障参与阿尔茨海默病的神经退行性过程。
注：以上文献为示例，非真实存在。实际研究中建议通过PubMed或Google Scholar检索真实文献。
CCL11 (C-C motif chemokine ligand 11), also known as eotaxin-1. is a small cytokine belonging to the CC chemokine family. It plays a critical role in recruiting eosinophils and other immune cells to sites of inflammation by interacting with its primary receptor CCR3. Initially identified for its involvement in allergic responses, CCL11 is implicated in conditions like asthma, atopic dermatitis, and eosinophilic disorders. Beyond immunology, emerging research links CCL11 to neuroinflammation and age-related cognitive decline, with elevated levels observed in Alzheimer’s disease and aging brains, earning it the informal label "the aging chemokine."
Recombinant CCL11 protein is produced using engineered bacterial (e.g., E. coli) or mammalian expression systems to ensure high purity and bioactivity. The non-glycosylated form, typically around 8-10 kDa, retains functional properties despite lacking post-translational modifications. Its applications span in vitro studies to investigate chemotaxis, immune cell signaling, and inflammatory pathways, as well as in vivo models of allergic diseases or neurological disorders. Researchers also utilize it to explore therapeutic interventions targeting CCR3-mediated pathways. Quality-controlled batches are essential for reproducibility, often validated through cell migration assays and receptor-binding tests. As a potential biomarker, recombinant CCL11 aids in standardizing diagnostic assays for clinical research. Its dual role in immunity and neurodegeneration continues to drive interdisciplinary interest in both therapeutic and mechanistic studies.
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