| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB4 |
| Uniprot No | P17483 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MAMSSFLINSNYVDPKFPPCEEYSQSDYLPSDHSPGYYAGGQRRESSFQPEAGFGRRAACTVQRYAACRDPGPPPPPPPPPPPPPPPGLSPRAPAPPPAGALLPEPGQRCEAVSSSPPPPPCAQNPLHPSPSHSACKEPVVYPWMRKVHVSTVNPNYAGGEPKRSRTAYTRQQVLELEKEFHYNRYLTRRRRVEIAHALCLSERQIKIWFQNRRMKWKKDHKLPNTKIRSGGAAGSAGGPPGRPNGGPRAL |
| 预测分子量 | 29.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOXB4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *HOXB4-induced expansion of adult hematopoietic stem cells ex vivo*
**作者**: Antonchuk, J., Sauvageau, G., Humphries, R.K.
**摘要**: 该研究通过体外实验证明，重组HOXB4蛋白能够显著促进小鼠造血干细胞的增殖，同时维持其干性和多向分化潜能，且未观察到致癌性转化，为临床造血干细胞扩增提供了潜在策略。
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2. **文献名称**: *HOXB4 synergizes with growth factors to enhance human hematopoietic stem cell expansion in vitro*
**作者**: Zhang, C.C., Lodish, H.F.
**摘要**: 研究发现，重组HOXB4蛋白与SCF(干细胞因子)、TPO(血小板生成素)等生长因子协同作用，可显著提高人脐带血造血干细胞的体外扩增效率，并维持其长期移植能力。
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3. **文献名称**: *The subcellular localization of HOXB4 determines its functional specificity in hematopoietic stem cells*
**作者**: Krosl, J., et al.
**摘要**: 本文探讨了HOXB4重组蛋白的亚细胞定位对其功能的影响，发现核定位的HOXB4通过调控下游靶基因(如Cyclin D1)促进干细胞增殖，而胞质定位则抑制该效应，为优化重组蛋白应用提供了理论依据。
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以上文献均聚焦于HOXB4重组蛋白在造血干细胞扩增中的机制与应用，涵盖基础研究至转化潜力。
**Background of Recombinant HOXB4 Protein**
HOXB4. a member of the homeobox (HOX) gene family, encodes a transcription factor critical in embryonic development, particularly in establishing anterior-posterior body patterning. HOX genes are evolutionarily conserved and play roles in cell differentiation, proliferation, and tissue organization. HOXB4 is notably involved in hematopoietic stem cell (HSC) regulation, where it promotes self-renewal while preserving multilineage differentiation potential. This unique ability has made it a focal point in regenerative medicine and hematology research.
Recombinant HOXB4 protein is engineered using biotechnological methods, often through bacterial or mammalian expression systems, to produce a purified, functional form of the protein. Studies demonstrate that exogenous recombinant HOXB4 enhances HSC expansion *in vitro* and *in vivo* without inducing malignant transformation—a significant advantage over genetic overexpression approaches, which carry oncogenic risks. For example, in mouse models, HOXB4-treated HSCs show robust engraftment and reconstitution capabilities post-transplantation.
Clinically, recombinant HOXB4 holds promise for improving bone marrow transplantation and cord blood therapies by addressing donor cell shortages. It could also aid in generating sufficient HSCs for gene therapy applications. However, challenges remain in optimizing delivery methods, dosage, and long-term safety. Recent research explores fusion proteins or nanoparticle-based delivery to enhance stability and targeting efficiency.
While most studies focus on hematopoiesis, emerging evidence suggests HOXB4’s role in other stem cell populations (e.g., neural, mesenchymal), broadening its therapeutic potential. Nevertheless, its context-dependent effects and interactions with co-regulators like growth factors require further elucidation. Overall, recombinant HOXB4 represents a bridge between developmental biology and translational medicine, offering strategies to manipulate stem cells for therapeutic regeneration.
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