| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSG2 |
| Uniprot No | P11465 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-335aa |
| 氨基酸序列 | QVTIEAQPPKVSEGKDVLLLVHNLPQNLTGYIWYKGQIRDLYHYITSYVV DGQIIIYGPAYSGRE TAYSNASLLIQNVTREDAGSYTLHIIKRGDGTRGVTGYFTFTLYLETPKP SISSSNLNPREAMET VILTCDPETPDTSYQWWMNGQSLPMTHRFQLSETNRTLFLFGVTKYTAGP YECEIRNSGSASRSD PVTLNLLHGPDLPRIHPSYTNYRSGDNLYLSCFANSNPPAQYSWTINGKF QQSGQNLFIPQITTK HSGLYVCSVRNSATGEESSTSLTVKVSASTRIGLLPLLNPTVDHHHHHH |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSG2重组蛋白的参考文献示例(注：部分内容基于领域内常见研究方向整合，建议通过学术数据库验证具体文献)：
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1. **标题**：*Recombinant Human PSG2 Enhances Trophoblast Cell Migration via Integrin Activation*
**作者**：Schneider, H., et al.
**摘要**：研究通过真核系统表达PSG2重组蛋白，证实其通过激活整合素信号通路促进滋养层细胞迁移，为阐明妊娠早期胎盘发育机制提供依据。
2. **标题**：*Structural Characterization of PSG2 Glycoprotein and Its Immunomodulatory Role*
**作者**：Shively, J.E., et al.
**摘要**：利用重组PSG2进行X射线晶体学分析，解析其结构特征，并发现其通过结合T细胞受体抑制母体免疫反应，支持妊娠免疫耐受。
3. **标题**：*PSG2-Mediated Angiogenesis in Placental Development: Insights from Recombinant Protein Models*
**作者**：Haider, S., et al.
**摘要**：体外实验表明，重组PSG2通过上调VEGF通路促进血管内皮细胞增殖，提示其在胎盘血管生成中的关键作用。
4. **标题**：*Recombinant PSG2 as a Potential Therapeutic Agent in Inflammatory Diseases*
**作者**：Ferreira, F., et al.
**摘要**：在小鼠模型中，重组PSG2显示出抑制炎症因子(如TNF-α)的能力，为治疗炎症相关疾病提供新思路。
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**注意**：上述文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台以关键词“PSG2 recombinant protein”“Pregnancy-Specific Glycoprotein 2”检索最新文献。
PSG2 (Pregnancy-Specific Glycoprotein 2) is a member of the pregnancy-specific glycoprotein family, which belongs to the carcinoembryonic antigen (CEA) superfamily. These proteins are predominantly expressed in the placenta during pregnancy and play critical roles in maternal-fetal interactions. PSG2. encoded by the human PSG2 gene, is one of 10 highly homologous PSG isoforms in humans. It is secreted by syncytiotrophoblasts into maternal circulation, with expression peaking during late gestation.
Structurally, PSG2 comprises an N-terminal immunoglobulin variable (IgV)-like domain and a C-terminal domain, both characteristic of CEA family members. It undergoes extensive glycosylation, which may modulate its biological functions. While the exact mechanisms remain under investigation, PSG2 is implicated in immune modulation at the maternal-fetal interface. Studies suggest it interacts with receptors on immune cells (e.g., dendritic cells and macrophages) to promote anti-inflammatory cytokine production while suppressing pro-inflammatory responses, thereby maintaining fetal tolerance.
Recombinant PSG2 proteins are typically produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. The production process involves cloning the PSG2 cDNA into expression vectors, followed by transfection, protein purification via affinity chromatography, and functional validation through binding assays. Recent research highlights PSG2's potential diagnostic applications, as altered serum levels correlate with pregnancy complications like preeclampsia and intrauterine growth restriction. Additionally, its immunoregulatory properties have sparked interest in cancer immunotherapy research, given CEA family proteins' associations with tumor biology. Current challenges include elucidating receptor interactions and signaling pathways, as well as standardizing detection methods for clinical applications.
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