| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein, NDFIP1, N4WBP5 |
| Entrez GeneID | 80762 |
| WB Predicted band size | 24.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NDFIP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NDFIP1抗体的参考文献示例(内容基于公开研究整理，非真实文献)：
1. **"NDFIP1 regulates T-cell activation by controlling ubiquitination of LAT adaptor protein"**
- **作者**: Smith A, et al.
- **摘要**: 研究通过NDFIP1抗体进行免疫印迹和免疫沉淀实验，发现NDFIP1通过招募Nedd4家族泛素连接酶调控T细胞活化关键蛋白LAT的泛素化，从而抑制T细胞过度激活。
2. **"NDFIP1-mediated endosomal sorting of EGFR modulates cancer cell proliferation"**
- **作者**: Chen L, et al.
- **摘要**: 利用NDFIP1特异性抗体进行免疫荧光定位，揭示NDFIP1通过促进EGFR内体分选和溶酶体降解，抑制表皮生长因子信号通路，降低肿瘤细胞增殖能力。
3. **"NDFIP1 deficiency exacerbates neuronal death in Parkinson’s disease models"**
- **作者**: Wang Y, et al.
- **摘要**: 通过NDFIP1抗体检测帕金森病模型中蛋白表达，发现NDFIP1缺失导致神经细胞中异常蛋白聚集增加，加速多巴胺能神经元退化，提示其神经保护作用。
4. **"Structural insights into NDFIP1-ITCH interaction in ubiquitin-dependent trafficking"**
- **作者**: Gupta R, et al.
- **摘要**: 结合NDFIP1抗体的共聚焦成像和结构分析，阐明NDFIP1与泛素连接酶ITCH的结合机制，及其在膜蛋白运输和细胞稳态中的调控功能。
(注：以上为模拟内容，实际文献需通过学术数据库检索确认。)
NDFIP1 (Nedd4 Family Interacting Protein 1) is a membrane-associated adaptor protein involved in regulating ubiquitination-mediated protein trafficking and degradation. It interacts with Nedd4 family E3 ubiquitin ligases via its N-terminal PY motifs, facilitating the ubiquitination of target proteins such as transporters, receptors, and signaling molecules. NDFIP1 is implicated in diverse cellular processes, including endosomal sorting, autophagy, and response to oxidative stress. Dysregulation of NDFIP1 has been linked to neurological disorders (e.g., Parkinson’s disease), cancer, and immune responses. For instance, it modulates PTEN/AKT/mTOR signaling in tumors and influences dopamine transporter turnover in neurons.
NDFIP1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NDFIP1’s role in disease models or cellular pathways. Commercially available antibodies are typically raised against specific epitopes (e.g., human NDFIP1 amino acids 50-150) and validated for cross-reactivity in common model organisms (mouse, rat). Researchers also employ these antibodies to explore NDFIP1’s regulatory effects on ubiquitin ligases (e.g., Nedd4-1. Smurf2) and its involvement in stress adaptation or tumor suppression mechanisms. Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity due to potential homology with NDFIP2.
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