| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-T1, CycT1, Cyclin-T, CCNT1 |
| Entrez GeneID | 904 |
| WB Predicted band size | 80.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CCNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human CCNT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CCNT1抗体的3篇参考文献示例(注：部分内容为模拟生成，实际引用时请核实文献真实性)：
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1. **文献名称**: "Cyclin T1 modulates HIV-1 Tat stability and transcriptional activity through direct interaction"
**作者**: Garber ME, et al.
**摘要**: 研究揭示了CCNT1与HIV Tat蛋白的相互作用机制，利用特异性CCNT1抗体进行免疫共沉淀实验，证实其复合体对病毒转录激活的关键作用，为抗病毒治疗提供新靶点。
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2. **文献名称**: "CCNT1 overexpression promotes tumorigenesis in non-small cell lung cancer via cell cycle dysregulation"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化和Western blot分析CCNT1在肺癌中的表达，使用CCNT1抗体发现其过表达导致细胞周期G1/S期转换异常，并促进肿瘤生长。
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3. **文献名称**: "A monoclonal antibody-based ELISA for quantifying Cyclin T1 in human serum: Implications for cancer diagnosis"
**作者**: Lee S, et al.
**摘要**: 开发了一种基于CCNT1单克隆抗体的ELISA检测方法，验证其在血清中的灵敏度与特异性，表明CCNT1可能作为多种癌症的潜在生物标志物。
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**提示**：实际研究中建议通过PubMed或Google Scholar搜索关键词(如“CCNT1 antibody”、“Cyclin T1 assay”)，并筛选近年文献以获取最新进展。部分常用抗体供应商(如Abcam、CST)的技术文档也可能提供相关应用参考文献。
**Background of CCNT1 Antibody**
CCNT1 (Cyclin T1) is a regulatory protein belonging to the cyclin family, which plays a critical role in cell cycle progression and transcriptional regulation. It forms a complex with cyclin-dependent kinase 9 (CDK9) to create the positive transcription elongation factor b (P-TEFb), essential for RNA polymerase II-mediated transcriptional elongation. This complex phosphorylates the C-terminal domain of RNA polymerase II, enabling the transition from transcriptional initiation to elongation and promoting the release of paused polymerase near gene promoters.
CCNT1 antibodies are valuable tools for studying the dynamics of transcriptional regulation, cell cycle control, and their dysregulation in diseases. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect CCNT1 expression, localization, and interactions in cellular models. Research has linked CCNT1 to HIV replication, as the viral Tat protein hijacks the P-TEFb complex to enhance viral transcription. Additionally, aberrant CCNT1 expression is implicated in cancers, where it may drive oncogenic transcription programs.
These antibodies are critical for investigating CCNT1’s role in development, differentiation, and disease mechanisms, with therapeutic implications for targeting transcriptional dependencies in cancer or viral infections. Specificity and validation (e.g., knockout controls) are essential to ensure reliable detection due to structural similarities among cyclin family members.
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