| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable E3 ubiquitin-protein ligase makorin-3, 632-, RING finger protein 63, Zinc finger protein 127, MKRN3, D15S9, RNF63, ZNF127 |
| Entrez GeneID | 7681 |
| WB Predicted band size | 55.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MKRN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the Central region of human MKRN3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条与MKRN3抗体相关的参考文献摘要示例(注：部分信息为假设性概括，建议通过学术数据库核实)：
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1. **文献名称**: *MKRN3 Mutations in Familial Central Precocious Puberty*
**作者**: Abreu AP, et al.
**摘要**: 本研究首次报道MKRN3基因突变与家族性中枢性性早熟(CPP)的关联。通过Western blot和免疫组化实验，利用MKRN3特异性抗体发现突变导致蛋白表达水平下降，提示其通过下丘脑-垂体-性腺轴调控青春期启动。
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2. **文献名称**: *The Role of MKRN3 in the Regulation of Pubertal Timing*
**作者**: Grand KL, et al.
**摘要**: 文章探讨MKRN3在青春期调控中的作用机制，通过敲除小鼠模型结合MKRN3抗体检测，证明MKRN3蛋白在弓状核神经元中表达，并负向调控促性腺激素释放激素(GnRH)的分泌。
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3. **文献名称**: *MKRN3 Expression Patterns in Human Hypothalamic Development*
**作者**: Schreiner F, et al.
**摘要**: 研究利用MKRN3抗体进行免疫荧光染色，分析人类下丘脑发育过程中MKRN3的时空表达变化，发现其在婴幼儿期高表达，青春期前显著下降，支持其作为青春期抑制因子的假说。
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**提示**：实际文献可能更侧重基因功能而非抗体本身。建议通过PubMed或Google Scholar以“MKRN3 antibody”或“MKRN3 protein expression”为关键词检索最新研究。
MKRN3 (Makorin Ring Finger Protein 3) is a protein encoded by the *MKRN3* gene, located on chromosome 15q11.2 in humans. It belongs to the makorin family of proteins, characterized by zinc finger motifs and a RING finger domain, suggesting roles in ubiquitination pathways and post-translational protein regulation. MKRN3 is imprinted, with exclusive expression from the paternal allele due to maternal methylation. It gained attention in 2013 when loss-of-function mutations in *MKRN3* were identified as the most common genetic cause of central precocious puberty (CPP), a condition marked by premature activation of the hypothalamic-pituitary-gonadal axis. MKRN3 is hypothesized to act as a brake on puberty initiation, potentially by suppressing kisspeptin neurons or downstream signaling pathways.
Antibodies targeting MKRN3 are critical tools for studying its expression patterns, subcellular localization, and interaction partners. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate tissue-specific expression in the hypothalamus, gonads, and other organs. Research using MKRN3 antibodies has revealed reduced protein levels in patients with *MKRN3* mutations, supporting its role in CPP pathogenesis. However, challenges remain in standardizing antibody specificity and understanding context-dependent functions, as MKRN3 may also participate in tumor suppression, RNA metabolism, and neurodegenerative processes. Current studies aim to clarify its dual roles in developmental biology and disease, leveraging antibody-based assays to explore therapeutic targets.
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