| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox even-skipped homolog protein 2, EVX-2, Evx2, Evx-2 |
| Entrez GeneID | 14029 |
| WB Predicted band size | 47.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Evx2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-107 amino acids from the N-terminal region of human Evx2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Evx2 (N-term)抗体的3篇参考文献的简要整理(注：因Evx2研究相对有限，部分文献可能涉及抗体应用或功能研究，以下内容基于假设性文献示例)：
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1. **文献名称**：*The role of Evx2 in limb development and its regulation by Sonic Hedgehog signaling*
**作者**：Smith A, et al.
**摘要**：本研究利用Evx2 N端抗体(抗体编号：AB123)通过免疫组化技术，在小鼠胚胎肢体芽中检测到Evx2蛋白的梯度表达，证实其在肢体极性形成中的关键作用。抗体特异性通过基因敲除小鼠模型验证。
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2. **文献名称**：*EVX2 as a potential oncogene in colorectal cancer: Expression and functional analysis*
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫荧光(使用抗Evx2 N-term抗体，货号sc-5678)，研究发现EVX2在结直肠癌组织中高表达，并与患者预后不良相关。抗体特异性通过siRNA敲减实验确认。
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3. **文献名称**：*Characterization of Evx2 antibody specificity for chromatin immunoprecipitation (ChIP) assays*
**作者**：Johnson R, et al.
**摘要**：本文系统评估了Evx2 N端抗体(来源：兔多克隆抗体)在ChIP实验中的适用性，成功鉴定了EvX2在胚胎干细胞分化过程中调控的靶基因。实验数据表明该抗体具有高亲和力和低交叉反应性。
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**说明**：实际文献需通过数据库(如PubMed、Google Scholar)以关键词“Evx2 antibody”“Evx2 N-terminal”检索。若研究稀缺，可扩展至相关基因(如Evx1)或联系抗体生产商(如Abcam、Santa Cruz)获取技术文档或引用文献。
The EVX2 (N-term) antibody is a targeted immunological tool designed to detect the N-terminal region of the Even-Skipped Homeobox 2 (EVX2) protein, a transcription factor encoded by the *EVX2* gene. EVX2 belongs to the homeobox gene family, which plays critical roles in embryonic development, particularly in pattern formation and cell differentiation. In vertebrates, EVX2 is implicated in regulating limb development and neurogenesis, with studies linking its dysregulation to developmental disorders and certain cancers. The N-terminal domain of EVX2 contains conserved structural motifs essential for DNA binding and protein-protein interactions, making it a focal region for functional studies.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess EVX2 expression levels, cellular localization, and tissue distribution. Its specificity for the N-terminal region ensures recognition of full-length or truncated EVX2 isoforms, aiding in the study of post-translational modifications or mutations affecting this domain. Researchers often validate the antibody using EVX2-overexpressing cell lines or knockout controls to confirm minimal cross-reactivity with homologous proteins like EVX1. Applications span developmental biology, regenerative medicine, and oncology, particularly in exploring EVX2's role in disease mechanisms or tissue regeneration. Commercial versions are typically raised in rabbits or mice, with quality checks for sensitivity and specificity. Proper controls are recommended to ensure accurate interpretation, given potential tissue-specific variations in EVX2 expression.
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