| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GLYA; SHMT; HEL-S-51e |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human SHMT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RSPO2抗体的3篇文献概括(虚构示例，仅供参考)：
1. **文献名称**：*RSPO2 as a Novel Biomarker in Colorectal Cancer: Expression Analysis Using a Specific Polyclonal Antibody*
**作者**：Kim et al.
**摘要**：研究通过开发兔源RSPO2多克隆抗体，证实RSPO2在结直肠癌组织中高表达，并与患者预后不良相关，提示其作为潜在诊断标志物的价值。
2. **文献名称**：*Targeting RSPO2-Wnt Signaling in Stem Cell Maintenance via Neutralizing Antibodies*
**作者**：Zhang et al.
**摘要**：利用单克隆抗体阻断RSPO2与Wnt受体结合，发现可抑制肠道干细胞增殖，为治疗Wnt依赖性肿瘤提供新策略。
3. **文献名称**：*RSPO2 Antibody-based Detection in Triple-Negative Breast Cancer: Implications for Therapeutic Targeting*
**作者**：Smith et al.
**摘要**：通过免疫组化验证RSPO2在三阴性乳腺癌中的特异性表达，并证明其抗体偶联药物在体外实验中显著抑制肿瘤细胞生长。
(注：以上文献为示例，实际文献需通过PubMed/Google Scholar等平台检索。)
The RSPO2 antibody is a tool used to detect R-spondin 2 (RSPO2), a secreted protein belonging to the R-spondin family, which comprises four members (RSPO1-4). RSPO2 plays a critical role in modulating the Wnt/β-catenin signaling pathway by binding to leucine-rich repeat-containing G-protein-coupled receptors (LGR4-6) and enhancing Wnt ligand activity. This pathway is essential for embryonic development, tissue homeostasis, stem cell maintenance, and cancer progression. RSPO2 is particularly implicated in organogenesis, angiogenesis, and tumorigenesis, with dysregulation linked to cancers, fibrosis, and developmental disorders.
The RSPO2 antibody is typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It is widely utilized in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to study RSPO2 expression, localization, and function in cells, tissues, or biological fluids. Validated antibodies exhibit specificity for RSPO2. avoiding cross-reactivity with other R-spondin family members.
Research applications include investigating RSPO2's role in Wnt-driven cancers (e.g., colorectal, breast), its interaction with tumor microenvironments, and its potential as a therapeutic target. Additionally, the antibody aids in developmental biology studies, particularly in limb and lung development. Commercial RSPO2 antibodies often undergo validation using knockout controls or siRNA-mediated silencing to confirm target specificity. Its clinical relevance extends to biomarker discovery and evaluating RSPO2 expression in disease models or patient samples.
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