| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Putative histone-lysine N-methyltransferase PRDM6, PR domain zinc finger protein 6, PR domain-containing protein 6, PRDM6, PFM3 |
| Entrez GeneID | 93166 |
| WB Predicted band size | 64.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PRDM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-72 amino acids from the N-terminal region of human PRDM6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRDM6 (N-term)抗体的参考文献示例(注：部分文献信息为模拟概括，供参考)：
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1. **文献名称**：*PRDM6 is required for vascular smooth muscle cell epigenetic homeostasis and development*
**作者**：Wu, F., et al.
**摘要**：研究揭示了PRDM6通过N端结构域介导组蛋白甲基化修饰，调控血管平滑肌细胞表观遗传程序和胚胎发育。文中使用PRDM6 (N-term)抗体进行ChIP-seq和免疫印迹实验，验证其在染色质结合中的功能。
2. **文献名称**：*A PRDM6-directed transcriptional complex coordinates neural crest differentiation*
**作者**：Park, S., et al.
**摘要**：该研究通过PRDM6 (N-term)抗体的免疫荧光和Co-IP实验，证明PRDM6的N端参与形成转录复合物，调控神经嵴细胞分化和迁移，为发育异常疾病提供机制解释。
3. **文献名称**：*Antibody validation and application of PRDM6-specific reagents in epigenetic studies*
**作者**：Zhang, L., et al.
**摘要**：文章详细描述了PRDM6 (N-term)抗体的制备与验证(包括肽段免疫、Western blot和免疫组化特异性检测)，并展示其在多种组织样本中检测PRDM6蛋白表达的应用。
4. **文献名称**：*PRDM6 modulates TGF-β signaling in cardiac fibrosis via N-terminal interactions*
**作者**：Kawakami, E., et al.
**摘要**：研究利用PRDM6 (N-term)抗体进行免疫沉淀和质谱分析，发现其N端与TGF-β信号通路蛋白互作，影响心脏纤维化进程，为治疗提供了潜在靶点。
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(注：以上文献为示例，实际引用需以真实发表论文为准。)
The PRDM6 (PR domain-containing protein 6) antibody targeting the N-terminal region is a key tool for studying the biological functions of this transcription regulator. PRDM6 belongs to the PRDM protein family, characterized by a conserved PR (PRDI-BF1-RIZ1 homologous) domain with potential histone methyltransferase activity. The N-terminal region of PRDM6 contains critical functional domains involved in protein-protein interactions and chromatin remodeling, making antibodies against this region valuable for detecting full-length or truncated isoforms. PRDM6 plays a role in vascular development, smooth muscle cell differentiation, and epigenetic regulation, often acting as a transcriptional repressor by recruiting histone modifiers.
Studies using PRDM6 (N-term) antibodies have elucidated its tissue-specific expression patterns, particularly in cardiovascular and neuronal systems, and its dysregulation in diseases like hypertension and cancer. This antibody is widely applied in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression levels, subcellular localization (primarily nuclear), and post-translational modifications. Validation via knockout controls or siRNA knockdown experiments is essential to confirm specificity, as PRDM6 shares structural homology with other PRDM family members. Ongoing research leverages this antibody to explore PRDM6's role in epigenetic pathways and its potential as a therapeutic target.
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