Rabbit Polyclonal MPP7(N-term) Antibody

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     All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution Lane 1: T47D whole cell lysate   Lane 2: Caco2 whole cell lysate                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 66kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution Lane 1: T47D whole cell lysate   Lane 2: Caco2 whole cell lysate Lane 3: A431 whole cell lysate                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated（ASP1615) at 1/10000 dilution. Observed band size : 66kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution Lane 1: T47D whole cell lysate   Lane 2: Caco2 whole cell lysate                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 66kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Flow cytometric analysis of Hela cells using MPP7 Antibody (N-term)(green, Cat#P34968) compared to an isotype control of rabbit IgG(blue).  P34968 was diluted at 1:25 dilution.  An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded H. kidney section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:100 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded H. liver section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:100 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded M. kidney section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

The MPP7 (N-term) antibody is designed to detect the N-terminal region of Membrane Protein, Palmitoylated 7 (MPP7), a member of the MAGUK (membrane-associated guanylate kinase) protein family. MPP7 plays a critical role in maintaining cell polarity and epithelial integrity by functioning as a scaffolding protein that organizes protein complexes at specialized cell junctions, such as tight junctions and adherens junctions. It interacts with other polarity regulators, including LIN7. DLG1. and CASK, to facilitate signaling and structural coordination during tissue development and homeostasis.

MPP7 is highly expressed in polarized epithelial tissues, including the kidney, liver, and intestine, and is implicated in diseases linked to epithelial dysfunction, such as cancer and renal disorders. The N-terminal domain of MPP7 is essential for its localization and interaction with binding partners, making antibodies targeting this region valuable tools for studying protein localization, complex formation, and functional mechanisms in cellular and pathological contexts.

Researchers utilize the MPP7 (N-term) antibody primarily in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression patterns, subcellular distribution, and involvement in signaling pathways. Validated applications often include studies of epithelial polarization, cell adhesion, and tissue morphogenesis, as well as exploring its potential role as a biomarker or therapeutic target in epithelial-derived cancers. Proper controls, such as knockout validation, are recommended to ensure specificity in experimental settings.