| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB |
| Entrez GeneID | 9217 |
| WB Predicted band size | 27.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This VAPB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 126-139 amino acids from the Central region of human VAPB. |
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以下是3篇与VAPB抗体相关的研究文献及其摘要概括：
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1. **文献名称**: *"VAPB mutations in ALS and related neurodegenerative disorders impair ER-mitochondria association and dynamics"*
**作者**: Teuling E. et al. (2008)
**摘要**: 研究揭示了VAPB蛋白突变(如P56S)与肌萎缩侧脊髓硬化症(ALS)的关联，发现突变导致内质网(ER)形态异常，并破坏其与线粒体、细胞骨架的相互作用。研究使用VAPB抗体检测突变蛋白的异常聚集。
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2. **文献名称**: *"ALS-linked protein VAPB regulates ER-mitochondria contact sites through interaction with PTPIP51"*
**作者**: Nishimura A.L. et al. (2014)
**摘要**: 通过VAPB抗体的免疫共沉淀实验，证实VAPB与线粒体相关蛋白PTPIP51直接结合，调控ER-线粒体接触，突变会损害钙离子稳态和细胞代谢，为ALS病理机制提供新视角。
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3. **文献名称**: *"Pathogenic VAPB protein aggregates in motor neurons occur through a metastable intermediate and disrupt mitochondrial function"*
**作者**: Mórotz G.M. et al. (2019)
**摘要**: 利用VAPB抗体进行免疫荧光分析，发现ALS相关突变VAPB蛋白在运动神经元中形成可溶性寡聚体，最终导致线粒体功能障碍和神经元死亡，揭示了疾病早期的分子病理特征。
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以上文献均涉及VAPB抗体在检测蛋白表达、定位及病理机制研究中的应用，聚焦于ALS等神经退行性疾病。如需具体实验方法或更完整引用，建议通过PubMed或Google Scholar检索标题获取全文信息。
The VAPB (Vesicle-associated Membrane Protein-associated Protein B) antibody is primarily used in research to detect and study the VAPB protein, a ubiquitously expressed endoplasmic reticulum (ER)-anchored protein involved in intracellular membrane trafficking, lipid transport, and ER-mitochondria/ER-Golgi contact site formation. VAPB plays a critical role in maintaining ER homeostasis, calcium signaling, and stress response. Mutations in the *VAPB* gene (e.g., P56S) are linked to neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). These mutations disrupt ER-mitochondria interactions, leading to protein misfolding, ER stress, and motor neuron degeneration.
VAPB antibodies are essential tools for investigating disease mechanisms, protein interactions, and subcellular localization in model systems. They are widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Additionally, autoantibodies targeting VAPB have been explored in autoimmune contexts, though their clinical relevance remains unclear. Research on VAPB antibodies contributes to understanding ALS pathogenesis and potential therapeutic strategies, such as modulating ER stress or enhancing protein quality control. Current studies also examine VAPB’s role in other conditions, including cancer and metabolic diseases, highlighting its broader cellular significance.
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