Rabbit Polyclonal MMlkl Antibody

MLKL (Mixed Lineage Kinase domain-Like) antibodies are essential tools for studying necroptosis, a form of regulated cell death distinct from apoptosis. MLKL is a key effector protein in the necroptosis pathway, activated via phosphorylation by RIPK3 (Receptor-Interacting Protein Kinase 3) in response to specific death signals, such as TNF-α or viral infection. This triggers MLKL oligomerization and translocation to the plasma membrane, disrupting membrane integrity and causing lytic cell death. Antibodies targeting MLKL are widely used to detect its expression, phosphorylation status (e.g., at Ser358/Thr357 in humans), and subcellular localization in experimental models. They enable researchers to investigate necroptosis involvement in diseases like inflammatory disorders, neurodegenerative conditions, and cancer, as well as evaluate therapeutic strategies targeting this pathway. Common applications include Western blotting, immunohistochemistry, and immunofluorescence. Specific MLKL antibodies may distinguish between inactive and activated (phosphorylated) forms, providing insights into pathway activation dynamics. Validation across species (human, mouse, rat) and sample types (cells, tissues) is critical for reliability. As necroptosis gains recognition in pathophysiology, MLKL antibodies remain pivotal for unraveling its mechanistic roles and translational potential.