| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AP-1 |
| Entrez GeneID | 3726; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JunB(Phospho-Ser259)抗体的3篇参考文献示例(内容为虚构，供参考)：
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1. **文献名称**：*Phosphorylation of JunB at Ser259 regulates AP-1 activity in T-cell signaling*
**作者**：Smith A, et al.
**摘要**：本研究揭示了JunB在T细胞活化过程中Ser259位点的磷酸化对AP-1转录复合物活性的调控作用。通过使用Phospho-Ser259特异性抗体，作者发现该修饰抑制JunB的核转位，从而下调IL-2表达，影响T细胞免疫应答。
2. **文献名称**：*A novel role of JunB phosphorylation in breast cancer metastasis*
**作者**：Chen L, et al.
**摘要**：文章利用JunB(Phospho-Ser259)抗体进行免疫沉淀和Western blot分析，证明该位点的磷酸化由ERK信号通路介导，并通过调控MMP-9表达促进乳腺癌细胞侵袭转移，为靶向治疗提供潜在标记物。
3. **文献名称**：*Development and validation of a phospho-specific antibody targeting JunB Ser259*
**作者**：Kim H, et al.
**摘要**：本研究报道了一种高特异性抗JunB(Phospho-Ser259)多克隆抗体的开发与验证。通过肽段免疫和亲和纯化，该抗体在ELISA、免疫荧光及ChIP实验中均表现出高灵敏度，可用于检测病理状态下JunB的动态磷酸化修饰。
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**说明**：以上文献为示例，实际引用需通过PubMed或学术数据库检索真实文献(如搜索关键词“JunB Ser259 phosphorylation antibody”)。若需具体文献，建议补充实验背景或应用场景以缩小范围。
JunB (Phospho-Ser259) antibody is a specialized tool used to detect the phosphorylated form of the JunB protein at serine residue 259. JunB, a member of the AP-1 (activator protein-1) transcription factor family, plays critical roles in regulating cellular processes such as proliferation, differentiation, apoptosis, and immune responses. Its activity is tightly controlled by post-translational modifications, including phosphorylation, which modulate its stability, subcellular localization, and DNA-binding capacity. Phosphorylation at Ser259 has been implicated in regulating JunB’s interaction with other AP-1 components and its ability to bind target gene promoters. This modification is often associated with signaling pathways involving mitogen-activated protein kinases (MAPKs) or other stress-responsive kinases.
The JunB (Phospho-Ser259) antibody is widely used in research to study the dynamics of JunB activation under various physiological or pathological conditions, such as inflammation, cancer, or cellular stress. It enables the detection of phosphorylated JunB in techniques like Western blotting, immunofluorescence, or immunohistochemistry, providing insights into pathway activation states. Studies have linked aberrant JunB phosphorylation to diseases like leukemia or solid tumors, highlighting its potential as a biomarker or therapeutic target. Researchers often validate this antibody’s specificity using knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals.
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