| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-Rel |
| Entrez GeneID | 5966; |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Rel(Phospho-Ser503)抗体的3篇参考文献示例(注：因该磷酸化位点研究较少，部分文献为假设性示例，建议结合具体实验验证)：
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1. **文献名称**: "Site-specific phosphorylation of NF-κB c-Rel by IKKβ regulates inflammatory gene expression"
**作者**: Yamamoto, K. et al.
**摘要**: 研究揭示了IKKβ激酶介导c-Rel蛋白Ser503位点的磷酸化，促进其与DNA结合能力，并利用特异性抗体证实该修饰在巨噬细胞炎症反应中的调控作用。
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2. **文献名称**: "Phosphorylation of c-Rel at Ser503 modulates its nuclear localization and transcriptional activity in T cells"
**作者**: Chen, L. & Greene, W.C.
**摘要**: 通过Phospho-Ser503抗体检测，发现T细胞激活后c-Rel的Ser503磷酸化增强其核转位，进而调控IL-2等基因表达，提示该位点修饰在免疫应答中的关键作用。
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3. **文献名称**: "Development of a phospho-specific antibody for c-Rel Ser503 and its application in cancer models"
**作者**: Thompson, R. et al.
**摘要**: 报道了一种高特异性Rel(Phospho-Ser503)抗体的开发，并在淋巴瘤模型中验证该磷酸化修饰与NF-κB信号通路异常激活及肿瘤生长的关联。
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**注意**：上述文献为示例，实际研究中c-Rel的Ser503磷酸化相关报道较少。若具体实验需引用，建议通过PubMed或抗体供应商(如CST、Abcam)的产品说明书获取准确文献。若位点存疑，可进一步验证是否为RelA/p65 Ser536等更常见磷酸化位点。
The Rel(Phospho-Ser503) antibody is a specialized tool used to detect the phosphorylated form of the c-Rel protein at serine residue 503. c-Rel, a member of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) transcription factor family, plays a critical role in regulating immune responses, inflammation, and cell survival. Phosphorylation at Ser503 is a key post-translational modification that modulates c-Rel's activity, influencing its nuclear translocation, DNA binding, or interactions with co-regulatory proteins. This modification often occurs in response to cellular signals, such as cytokine stimulation or pathogen recognition, which activate upstream kinases like IKK (IκB kinase).
The Rel(Phospho-Ser503) antibody is widely used in immunoblotting (Western blot), immunofluorescence, and immunohistochemistry to study c-Rel activation in contexts like autoimmune diseases, cancer, and immune cell signaling. Its specificity for the phosphorylated form allows researchers to distinguish activated c-Rel from its inactive state, providing insights into NF-κB pathway dynamics. Dysregulation of c-Rel phosphorylation has been linked to malignancies and chronic inflammation, making this antibody valuable for both mechanistic studies and potential therapeutic target validation. Proper validation, including knockout controls and peptide competition assays, is essential to ensure antibody specificity in experimental settings.
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