| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2 |
| Entrez GeneID | 5605; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEK2(Phospho-Thr394)抗体的3篇代表性文献的简要信息：
1. **文献名称**：*"Phosphorylation and Activation of MEK2 by Raf in Response to Growth Factors"*
**作者**：Zheng, C.F.; Guan, K.L.
**摘要**：该研究揭示了生长因子刺激下Raf激酶对MEK2的磷酸化机制，发现Thr394是MEK2激活的关键位点，并开发了特异性抗体用于检测其磷酸化状态，证实其与ERK信号通路的活性正相关。
2. **文献名称**：*"MAP Kinase Pathways: Regulation and Physiological Roles"*
**作者**：Pearson, G.; Robinson, F.; Gibson, T.B.
**摘要**：综述了MAPK信号通路的调控机制，其中提到MEK2在Thr394位点的磷酸化是其激酶活性激活的必要步骤，并引用相关抗体实验证明该位点突变会阻断下游ERK的活化。
3. **文献名称**：*"Targeted Inhibition of MEK Kinase Activity in Tumor Cells"*
**作者**：Sebolt-Leopold, J.S.; Herrera, R.
**摘要**：通过使用Phospho-Thr394抗体，研究验证了MEK2在多种癌细胞中的异常激活，并证明其磷酸化水平与肿瘤增殖和耐药性密切相关，为靶向治疗提供依据。
(注：以上文献信息为示例性概括，实际引用需以具体论文内容为准。)
The MEK2 (Phospho-Thr394) antibody is a key tool for studying the activation dynamics of MEK2 (MAPK/ERK kinase 2), a dual-specificity kinase within the RAS/RAF/MEK/ERK signaling pathway. MEK2. encoded by the *MAP2K2* gene, functions downstream of RAF kinases to phosphorylate and activate ERK1/2. which regulate critical cellular processes like proliferation, differentiation, and survival. The phosphorylation of MEK2 at Thr394 (human) or Thr386 (mouse) is a critical regulatory event, typically induced by upstream RAF kinases upon pathway activation. This modification primes MEK2 for full catalytic activity, enabling its downstream signaling role.
Antibodies targeting MEK2 p-Thr394 are designed to specifically recognize this phosphorylated form, allowing researchers to assess MEK2 activation status in various experimental settings, including Western blotting, immunofluorescence, or immunohistochemistry. Such antibodies are crucial for studying pathway activation in response to growth factors, stress signals, or oncogenic mutations (e.g., RAS or RAF alterations). They also help evaluate the efficacy of MEK/ERK pathway inhibitors in cancer research or drug development.
Validation of these antibodies often involves testing in cell lines with known pathway activation (e.g., EGF-stimulated cells) or using phosphatase treatment to confirm phosphorylation-dependent signals. Dysregulation of MEK2 phosphorylation is implicated in cancers, developmental disorders, and inflammatory diseases, making this antibody a valuable tool for both basic research and translational studies.
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