| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ELK1; ETS-domain protein Elk-1; |
| Entrez GeneID | 2002; |
| WB Predicted band size | 47kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与Elk-1 (Phospho-Ser383)抗体相关的文献摘要概括：
---
1. **文献名称**: "Phosphorylation of transcription factor Elk-1 by MAP kinase is required for mitogen-induced c-fos expression"
**作者**: Gille, H. et al.
**摘要**: 研究证实丝裂原激活蛋白激酶(MAPK)介导的Elk-1在Ser383位点的磷酸化是其转录激活的关键步骤，该抗体用于Western blot验证磷酸化修饰，证明其在调控c-fos基因表达中的必要性。
2. **文献名称**: "Neuronal activity-dependent phosphorylation of Elk-1 in the hippocampus"
**作者**: Yang, S.H. et al.
**摘要**: 通过免疫组化和Western blot分析，发现神经元活动中Elk-1的Ser383磷酸化与CREB协同调控突触可塑性相关基因，该抗体被用于定位海马区磷酸化Elk-1的动态变化。
3. **文献名称**: "Regulation of transcription factor function by phosphorylation"
**作者**: Sharrocks, A.D. et al.
**摘要**: 综述中讨论了Elk-1的Ser383磷酸化如何通过改变其与DNA和共激活因子的结合能力调控转录活性，引用该抗体的实验数据说明其在信号转导研究中的应用。
---
这些文献涵盖了Elk-1 (Phospho-Ser383)抗体在机制研究、神经科学及转录调控中的典型应用。如需具体实验细节，建议查阅全文或相关抗体产品说明书。
The Elk-1 (Phospho-Ser383) antibody is a key tool for studying the activation status of the Elk-1 transcription factor, a member of the ternary complex factor (TCF) subfamily. Elk-1 regulates gene expression by binding to serum response elements (SREs) in promoter regions, often in conjunction with serum response factor (SRF). Its activity is tightly controlled by phosphorylation, particularly at Serine 383 (Ser383), a critical residue within the transcriptional activation domain. Phosphorylation at this site is primarily mediated by mitogen-activated protein kinases (MAPKs), such as ERK, JNK, or p38. in response to extracellular stimuli like growth factors, stress signals, or mitogens. This post-translational modification enhances Elk-1’s transcriptional activity, enabling it to drive the expression of target genes involved in cell proliferation, differentiation, and apoptosis.
The Elk-1 (Phospho-Ser383) antibody specifically detects Elk-1 when phosphorylated at Ser383. making it invaluable for assessing MAPK pathway activation in cellular signaling studies. It is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate context-dependent signaling events in cancer, neurodegeneration, or immune responses. Researchers rely on this antibody to explore dynamic phosphorylation changes under experimental conditions, providing insights into disease mechanisms or drug effects targeting MAPK/Elk-1 pathways. Proper validation, including knockout controls or phosphatase treatment, ensures specificity for accurate data interpretation.
×
