| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta |
| Entrez GeneID | 2932; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于 **GSK3β(Phospho-Ser9)抗体** 的3篇参考文献，简要概括如下：
---
1. **文献名称**：*"Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B"*
**作者**：Cross, D.A., Alessi, D.R., Cohen, P., et al.
**摘要**：该研究首次揭示了胰岛素通过激活蛋白激酶B(PKB/Akt)诱导GSK3β Ser9位点的磷酸化，从而抑制其激酶活性，阐明了胰岛素信号通路中GSK3β的调控机制。
2. **文献名称**：*"GSK3 takes centre stage more than 20 years after its discovery"*
**作者**：Frame, S., Cohen, P.
**摘要**：综述文章系统总结了GSK3β的功能及其磷酸化调控(包括Ser9位点)，强调其在Wnt/β-catenin信号通路、细胞周期及疾病中的作用，并讨论了相关抗体在实验中的应用。
3. **文献名称**：*"Glycogen synthase kinase-3 (GSK3): regulation, actions, and diseases"*
**作者**：Beurel, E., Grieco, S.F., Jope, R.S.
**摘要**：本文详细探讨了GSK3β的磷酸化修饰(如Ser9)在神经退行性疾病、癌症和炎症中的病理意义，并总结了使用Phospho-Ser9抗体检测其在疾病模型中的活性变化。
---
以上文献均涉及GSK3β Ser9磷酸化的分子机制、功能或实验应用，相关抗体常用于检测其磷酸化状态以研究信号通路或疾病机制。如需具体实验方法，可参考抗体供应商(如CST、Abcam)的技术手册。
The GSK3β (Phospho-Ser9) antibody detects glycogen synthase kinase 3 beta (GSK3β) when phosphorylated at serine 9. a key regulatory modification that inhibits its kinase activity. GSK3β is a ubiquitously expressed serine/threonine kinase involved in diverse cellular processes, including metabolism, cell proliferation, differentiation, and apoptosis. Unlike most kinases, GSK3β is constitutively active in resting cells and is inactivated upon phosphorylation at Ser9. This phosphorylation is primarily mediated by upstream kinases such as AKT (Protein Kinase B), PKA, or PKC in response to signaling pathways like insulin/IGF-1. Wnt, or PI3K/AKT cascades.
The Phospho-Ser9 epitope serves as a critical marker for studying GSK3β regulation in physiological and pathological contexts. Dysregulation of GSK3β activity, linked to impaired Ser9 phosphorylation, is associated with diseases such as diabetes, Alzheimer's disease, cancer, and mood disorders. Researchers widely use this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess GSK3β inactivation in cell/tissue samples, drug response studies, or disease models. Its specificity for the phosphorylated form allows precise tracking of pathway activation, making it essential for investigating therapeutic targets modulating GSK3β activity.
×
