Rabbit Polyclonal TNNI2(N-term) Antibody

The TNNI2 (N-term) antibody is a specific immunological tool designed to target the N-terminal region of Troponin I type 2 (TNNI2), a protein encoded by the TNNI2 gene. TNNI2 is a skeletal muscle-specific isoform of troponin I, a critical component of the troponin complex that regulates calcium-dependent muscle contraction in fast-twitch skeletal muscle fibers. This antibody is commonly used in research applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect and quantify TNNI2 expression in tissues or cell lysates. The N-terminal region of TNNI2 is relatively conserved across species, making the antibody suitable for cross-reactivity studies in models like humans, mice, and rats. TNNI2 dysfunction or mutations have been implicated in congenital musculoskeletal disorders, such as distal arthrogryposis syndromes, characterized by impaired muscle development and contractility. Researchers utilize this antibody to investigate TNNI2's role in muscle physiology, regeneration, and disease mechanisms. Its specificity for the N-terminal domain helps distinguish TNNI2 from other troponin I isoforms (e.g., TNNI1 and TNNI3), ensuring accurate detection in studies focused on skeletal muscle-specific pathways. Validation typically includes testing in knockout models or siRNA-treated samples to confirm binding specificity.