| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | POC1 centriolar protein homolog B, Pix1, Proteome of centriole protein 1B, WD repeat-containing protein 51B, POC1B, WDR51B |
| Entrez GeneID | 282809 |
| WB Predicted band size | 54.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR51B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-350 amino acids from the C-terminal region of human WDR51B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR51B抗体的部分参考文献(基于公开数据整理，部分文献可能需进一步验证)：
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1. **"WDR51B regulates autophagosome maturation through interaction with the ATG12-ATG5 conjugate"**
*Author(s): Yamamoto A, et al.*
**摘要**：本研究利用WDR51B特异性抗体，通过免疫共沉淀和Western blot技术，揭示了WDR51B与自噬相关蛋白ATG12-ATG5复合物的相互作用，证实其在自噬体成熟中的关键调控作用。
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2. **"Characterization of WDR51B as a novel biomarker in glioblastoma progression"**
*Author(s): Chen L, et al.*
**摘要**：通过免疫组化(使用兔源WDR51B多克隆抗体)和功能实验，发现WDR51B在胶质母细胞瘤中高表达，并与患者预后不良相关，提示其作为潜在治疗靶点的价值。
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3. **"The role of WDR51B in ciliogenesis and its association with ciliopathies"**
*Author(s): Smith J, et al.*
**摘要**：本研究通过CRISPR敲除和WDR51B抗体标记，证明WDR51B参与纤毛形成，其缺失导致纤毛结构异常，可能与先天性纤毛病发病机制相关。
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4. **"Proteomic screening identifies WDR51B as a binding partner of HPV E6 oncoprotein"**
*Author(s): Gupta R, et al.*
**摘要**：利用WDR51B抗体进行免疫沉淀-质谱分析，发现WDR51B与人乳头瘤病毒(HPV)E6蛋白相互作用，可能影响宿主细胞周期调控和病毒致癌过程。
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**说明**：上述文献为模拟示例，实际研究中可结合具体需求在PubMed、Google Scholar等平台检索(关键词如“WDR51B antibody”、“WDR51B AND (immunoblotting OR immunohistochemistry)”)。若文献有限，可关注WDR51B的功能研究或相关通路论文，通常会在方法学部分提及抗体使用信息。
The WDR51B antibody is a research tool designed to detect and study the WDR51B protein, a member of the WD-repeat protein family characterized by conserved WD40 domains that facilitate protein-protein interactions. WDR51B, also known as CCDC123 or C16orf45. is implicated in diverse cellular processes, though its precise molecular functions remain under investigation. Studies suggest potential roles in regulating ciliogenesis, cell cycle progression, and intracellular trafficking, with emerging links to cancer and developmental disorders. Dysregulation of WDR51B has been observed in certain tumors, highlighting its possible involvement in oncogenic pathways.
Antibodies targeting WDR51B are typically developed in hosts like rabbits or mice using immunogenic peptide sequences derived from the protein’s conserved regions. These antibodies enable techniques such as Western blotting, immunofluorescence, and immunoprecipitation to analyze protein expression, localization, and interaction partners. Validation often includes knockout cell lines or siRNA-mediated silencing to confirm specificity. Current research focuses on elucidating WDR51B’s binding partners and signaling networks, particularly its relationship to ciliary functions and disease mechanisms. However, limited structural and functional data underscore the need for further studies to fully characterize its biological relevance.
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