| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase MmuIIIB, DNA MTase MmuIIIB, MMmuIIIB, Dnmt3b |
| Entrez GeneID | 13436 |
| WB Predicted band size | 97.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Dnmt3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 356-390 amino acids from the Central region of Mouse Dnmt3b. |
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以下是3篇关于小鼠Dnmt3b抗体的文献信息(基于模拟生成，非真实文献)：
1. **文献名称**: "Characterization of a monoclonal antibody specific to mouse Dnmt3b for epigenetic studies"
**作者**: Li Y, et al.
**摘要**: 该研究开发了一种针对小鼠Dnmt3b蛋白C端结构域的单克隆抗体，并通过Western blot和免疫荧光验证其特异性，成功应用于胚胎干细胞分化过程中Dnmt3b表达动态的检测。
2. **文献名称**: "Dnmt3b knockout mice reveal its essential role in neural crest development"
**作者**: Smith J, et al.
**摘要**: 利用特异性Dnmt3b抗体在敲除小鼠模型中验证蛋白缺失，证明Dnmt3b通过调控特定基因甲基化参与神经嵴细胞分化和颅面发育。
3. **文献名称**: "Tissue-specific expression of Dnmt3b isoforms in mouse embryos"
**作者**: Garcia R, et al.
**摘要**: 通过多克隆Dnmt3b抗体结合免疫组化，揭示了Dnmt3b不同剪切变体在小鼠胚胎组织(如心脏、肝脏)中的差异表达及功能。
4. **文献名称**: "Antibody validation for chromatin immunoprecipitation of Dnmt3b in murine cells"
**作者**: Kim H, et al.
**摘要**: 系统评估了三种商业小鼠Dnmt3b抗体在ChIP实验中的适用性，确认了其中一种抗体在染色质结合位点分析中的高特异性和低背景信号。
注：以上文献为模拟生成，实际引用需查询真实数据库(如PubMed)。
The mouse Dnmt3b antibody is a crucial tool for studying DNA methyltransferase 3B (DNMT3B), an enzyme essential for establishing de novo DNA methylation patterns during mammalian development. As part of the DNMT3 family, alongside DNMT3A and DNMT3L, DNMT3B plays a pivotal role in embryogenesis, germ cell differentiation, and genomic imprinting by catalyzing the transfer of methyl groups to cytosine residues in CpG dinucleotides. Unlike its paralog DNMT3A, DNMT3B exhibits unique expression dynamics, with peak activity during early embryogenesis and preferential targeting of pericentromeric repeats and gene bodies. Dysregulation of DNMT3B is linked to developmental disorders like immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome and cancer progression.
Mouse-specific Dnmt3b antibodies are designed to recognize endogenous DNMT3B protein in murine tissues or cell lines, enabling researchers to investigate its expression, localization, and interactions. These antibodies are commonly validated through techniques such as Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP). Given the existence of multiple DNMT3B isoforms generated by alternative splicing, antibody specificity for particular isoforms or post-translational modifications (e.g., phosphorylation) is a critical consideration. Such tools have advanced studies in epigenetic reprogramming, stem cell biology, and disease models, particularly in understanding how DNMT3B-mediated methylation impacts gene silencing, X-chromosome inactivation, and transposable element suppression. Proper validation using knockout controls remains essential due to potential cross-reactivity with DNMT3A or other epigenetic regulators.
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