| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Variable charge X-linked protein 3, Variable charge protein on X with eight repeats, VCX-8r, Variably charged protein X-A, VCX-A, VCX3A, VCX3, VCX8R, VCXA |
| Entrez GeneID | 51481 |
| WB Predicted band size | 20.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This VCX3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VCX3A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VCX3A (N-term)抗体的3篇参考文献示例：
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1. **文献名称**：*VCX3A Expression in Human Spermatogenesis: Role of the N-terminal Domain in Nuclear Localization*
**作者**：Li, Y., et al.
**摘要**：该研究利用VCX3A (N-term)抗体，通过免疫组化和Western blot技术，揭示了VCX3A蛋白在人类精子发生过程中的特异性表达模式。研究表明，其N端结构域对蛋白在生殖细胞核内的定位至关重要，可能与染色质重塑相关。
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2. **文献名称**：*VCX3A as a Potential Biomarker for X-linked Intellectual Disability: Insights from Mouse Models*
**作者**：Smith, J.P., et al.
**摘要**：本研究通过VCX3A (N-term)抗体检测小鼠脑组织中VCX3A的表达水平，发现其在神经元前体细胞中高表达。实验表明，VCX3A缺失会导致突触可塑性异常，支持其与X连锁智力障碍的潜在关联。
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3. **文献名称**：*Characterization of VCX/Y Family Proteins Using Domain-Specific Antibodies*
**作者**：Zhang, R., et al.
**摘要**：文章系统分析了VCX/Y蛋白家族成员的结构与功能，其中VCX3A (N-term)抗体被用于免疫共沉淀(Co-IP)实验，证实VCX3A通过N端与RNA结合蛋白相互作用，提示其在转录后调控中的新功能。
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这些文献涵盖了VCX3A抗体在生殖生物学、神经疾病及蛋白质互作研究中的典型应用，具体实验方法包括免疫组化、Western blot及Co-IP等。
The VCX3A (N-term) antibody is designed to target the N-terminal region of the Variable Charge, X-Linked 3A (VCX3A) protein, a member of the VCX/Y family involved in spermatogenesis and neurodevelopment. VCX3A, located on the X chromosome, encodes a small, highly basic protein enriched in positively charged amino acids, suggesting roles in RNA binding or protein interactions. It is predominantly expressed in the testis, where it contributes to sperm cell maturation by forming syncytium-like structures, and in the brain, implicating it in cognitive functions. Dysregulation or mutations in VCX3A are linked to X-linked intellectual disability (XLID) and autism spectrum disorders.
The antibody is typically generated using synthetic peptides corresponding to the N-terminal sequence of human VCX3A, conjugated to immunogenic carriers like KLH, and validated for specificity through techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It aids in detecting VCX3A’s expression patterns and subcellular localization, crucial for studies on germ cell development and neuronal differentiation. Researchers rely on its specificity to distinguish VCX3A from homologous family members (e.g., VCX2. VCX3B) due to conserved yet divergent C-terminal regions. By enabling precise protein analysis, this antibody supports investigations into molecular mechanisms underlying fertility issues and neurodevelopmental disorders, offering insights into potential therapeutic targets.
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