| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Melanophilin, Exophilin-3, Slp homolog lacking C2 domains a, SlaC2-a, Synaptotagmin-like protein 2a, MLPH, SLAC2A |
| Entrez GeneID | 79083 |
| WB Predicted band size | 65.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MLPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-569 amino acids from the C-terminal region of human MLPH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MLPH抗体的3篇参考文献示例(注：文献为虚构示例，仅用于格式演示)：
---
1. **"MLPH regulates melanosome transport via interaction with Rab27A and MyoVa in melanocytes"**
*Authors: Ménasché G, Pastural E*
摘要：研究通过免疫共沉淀和抗体标记实验，揭示了MLPH蛋白在黑色素体中作为Rab27A与Myosin Va(MyoVa)的衔接分子作用，调控黑色素颗粒的胞内运输机制。
2. **"Diagnostic utility of MLPH-specific antibodies in Griscelli syndrome type 3"**
*Authors: Sevilla LM, Ishida M*
摘要：本文评估了MLPH抗体在Griscelli综合征3型诊断中的应用，发现其能有效区分MLPH基因突变导致的色素异常与其他亚型，为临床提供可靠标记。
3. **"Comparative analysis of MLPH antibody specificity across vertebrate species"**
*Authors: Hiroaki N, Okamoto S*
摘要：通过Western blot和免疫组化，比较了多种MLPH抗体在小鼠、斑马鱼等模型中的交叉反应性，为跨物种黑色素运输研究提供抗体选择依据。
---
*注：以上文献信息为模拟内容，实际引用请查询PubMed、Web of Science等数据库获取真实论文。*
The MLPH (melanophilin) antibody is a crucial tool in studying melanosome transport and pigmentation disorders. MLPH encodes melanophilin, a protein that bridges RAB27A-bound melanosomes and myosin Va motor complexes, enabling their intracellular movement in melanocytes. This tripartite complex (RAB27A-MLPH-myosin Va) ensures proper melanosome distribution, critical for skin, hair, and eye pigmentation.
MLPH antibodies are widely used in immunohistochemistry, Western blotting, and immunofluorescence to detect melanophilin expression levels and localization. Research applications include investigating genetic mutations causing Griscelli syndrome type 3 (GS3), a rare autosomal recessive disorder characterized by hypopigmentation and silvery hair. MLPH mutations disrupt melanosome-keratinocyte transfer, linking directly to GS3 pathogenesis.
In oncology, MLPH antibodies help study melanoma progression, as altered melanosome transport mechanisms are implicated in cancer metastasis. Additionally, these antibodies aid in distinguishing pigmentation-related disorders from other vesicular transport diseases. Recent studies also explore MLPH's role beyond pigmentation, including immune cell vesicle trafficking and neurological functions, expanding its biomedical relevance. Commercial MLPH antibodies are typically validated in human and murine tissues, supporting translational research across species.
×
