Rabbit Polyclonal NFkB-p105/p50(Phospho-Ser337) Antibody

The NF-κB-p105/p50 (Phospho-Ser337) antibody is designed to detect the phosphorylated form of the NF-κB p105 precursor protein at serine residue 337. which corresponds to Ser337 in the human p105 (NFKB1 gene product). NF-κB is a family of transcription factors central to immune responses, inflammation, cell survival, and proliferation. The p105 protein serves as both a precursor for the p50 subunit (generated via proteolytic processing) and an IκB-like regulator of other NF-κB members. Phosphorylation at Ser337 occurs during the canonical NF-κB activation pathway, typically triggered by stimuli such as cytokines, pathogens, or stress. This modification facilitates the proteasomal processing of p105 into p50. enabling p50 to dimerize with Rel proteins (e.g., p65) and translocate to the nucleus to regulate target gene expression. The antibody specifically recognizes the phosphorylated Ser337 epitope, making it a valuable tool for studying NF-κB activation dynamics in pathways like TLR, TNF-α, or IL-1R signaling. Researchers use it in techniques such as Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation-dependent regulatory mechanisms in cellular models, tissue samples, or disease contexts (e.g., cancer, autoimmune disorders). Proper controls, including unphosphorylated samples or phosphatase-treated lysates, are essential to confirm specificity. This antibody aids in elucidating how post-translational modifications fine-tune NF-κB activity in health and disease.