| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p65, NFKB3, |
| Entrez GeneID | 5970; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NF-κB p65 (Phospho-Ser468)抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
1. **文献名称**：*Phosphorylation of NF-κB p65 at Ser468 controls its DNA binding and inflammatory function*
**作者**：Chen et al.
**摘要**：研究揭示了p65亚基Ser468位点的磷酸化通过抑制其与DNA结合能力，负调控NF-κB的转录活性，该抗体用于验证炎症模型中该修饰的动态变化。
2. **文献名称**：*Site-specific phosphorylation of NF-κB p65 by IKKε regulates virus-induced interferon production*
**作者**：Wang et al.
**摘要**：发现IKKε激酶介导的p65 Ser468磷酸化在抗病毒免疫应答中促进I型干扰素表达，抗体被用于免疫印迹和染色质免疫共沉淀(ChIP)实验。
3. **文献名称**：*Ser468 phosphorylation modulates NF-κB/p65 nuclear translocation in cancer cells*
**作者**：Li et al.
**摘要**：通过免疫荧光和蛋白质印迹分析，证明肿瘤微环境中p65 Ser468磷酸化通过影响核转位促进癌细胞存活，该抗体用于临床样本的病理学检测。
注：以上为虚构示例，实际文献需通过PubMed/Google Scholar检索关键词“NF-κB p65 Ser468 phosphorylation antibody”获取。
The NF-κB p65 (Phospho-Ser468) antibody is a specialized tool used to detect the phosphorylated form of the NF-κB p65 subunit at serine residue 468. NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a transcription factor family central to regulating immune responses, inflammation, cell survival, and proliferation. The p65 subunit (RelA) is a key component of the canonical NF-κB pathway. In resting cells, NF-κB complexes are sequestered in the cytoplasm by inhibitory IκB proteins. Upon stimulation (e.g., cytokines, pathogens, stress), IκB is phosphorylated and degraded, enabling NF-κB translocation to the nucleus to activate target genes.
Phosphorylation of p65 at specific serine residues, including Ser468. modulates its transcriptional activity, DNA binding, and interactions with co-regulators. Ser468 phosphorylation is associated with non-canonical signaling pathways and may be catalyzed by kinases such as IKKβ or others depending on cellular context. This post-translational modification can influence the duration and specificity of NF-κB-driven gene expression, impacting processes like apoptosis and immune regulation.
The NF-κB p65 (Phospho-Ser468) antibody is widely used in research to study NF-κB activation dynamics, particularly in diseases involving chronic inflammation, cancer, and autoimmune disorders. It enables detection of phosphorylation events via techniques like Western blotting, immunofluorescence, and immunohistochemistry, providing insights into pathway activation status. Validation of this antibody typically includes testing in knockout models or phosphatase-treated samples to ensure specificity for the phosphorylated epitope.
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