Rabbit Polyclonal NFkB-p65(Phospho-Thr254) Antibody

中文名： NFkB-p65(Phospho-Thr254)抗体
别名： NFKB3; RELA; TF65; Transcription factor p65; p65

货号: IPDX40010

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/50-1/100	Human,Mouse,Rat
ICC	1/100-1/200	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	NFKB3; RELA; TF65; Transcription factor p65; p65
Entrez GeneID	5970;
WB Predicted band size	65kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFkB-p65.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of extracts from 3T3 cells untreated or treated with TNF-a using NFkB-p65(Phospho-Thr254) Antibody P40010.
Immunofluorescence staining of methanol-fixed Hela using NFkB-p65(Phospho-Thr254) Antibody P40010.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65(Phospho-Thr254) Antibody P40010(left) or the same antibody preincubated with blocking peptide(right).

参考文献

以下是关于NF-κB p65 (Phospho-Thr254)抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过数据库检索确认)：

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1. **文献名称**：*Phosphorylation of RelA/p65 at Threonine 254 Regulates NF-κB-Dependent Gene Expression*

**作者**：Chen, L. et al.

**摘要**：本研究揭示了p65 Thr254位点的磷酸化在NF-κB信号通路中的关键作用。作者开发了一种特异性识别p65 Thr254磷酸化的抗体，并证实该修饰通过促进p65核转位增强促炎细胞因子(如TNF-α和IL-6)的表达。

2. **文献名称**：*A Novel Phospho-Specific Antibody Reveals TLR4-Induced Phosphorylation of NF-κB p65 on Thr254*

**作者**：Wang, Y. et al.

**摘要**：文章报道了一种新型抗p65 Thr254磷酸化抗体的制备与验证。通过免疫印迹和免疫荧光实验，作者证明TLR4激活后巨噬细胞中Thr254磷酸化水平显著升高，且该修饰与NF-κB的转录活性密切相关。

3. **文献名称**：*Threonine 254 Phosphorylation Modulates p65 Stability in Colorectal Cancer*

**作者**：Kim, S. et al.

**摘要**：本研究利用Phospho-Thr254特异性抗体，发现结直肠癌细胞中p65 Thr254的异常磷酸化通过抑制泛素化降解途径增强其蛋白稳定性，进而促进肿瘤细胞的增殖和转移。

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**注意事项**：

- 以上文献为模拟生成，实际研究中p65的常见磷酸化位点(如Ser536)可能更广泛被研究。

- 建议通过PubMed或Google Scholar以关键词“NF-κB p65 Thr254 phosphorylation antibody”检索真实文献，并确认位点编号的准确性(如是否为Thr253或Thr254)。

- 部分抗体厂商(如Cell Signaling Technology)的产品手册可能提供相关参考文献。

背景信息

The NF-κB-p65 (Phospho-Thr254) antibody is a specialized tool used to detect the phosphorylated form of the p65 subunit (RelA) of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcription factor at threonine 254. NF-κB is a critical regulator of immune response, inflammation, cell survival, and proliferation. The p65 subunit plays a central role in mediating NF-κB signaling by translocating to the nucleus upon activation, where it binds DNA to regulate target gene expression. Phosphorylation at Thr254 is a post-translational modification associated with the nuclear localization and transcriptional activity of p65. This modification can be induced by various stimuli, including pro-inflammatory cytokines (e.g., TNF-α, IL-1β) or cellular stress, and is tightly regulated by kinases such as IKK (IκB kinase) complex components or other upstream signaling molecules.

The antibody is widely used in research to study NF-κB activation dynamics in diseases like cancer, autoimmune disorders, and chronic inflammation. It enables the detection of phosphorylated p65 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, helping to elucidate signaling pathways, drug mechanisms, or disease biomarkers. Specificity for the phosphorylated Thr254 residue ensures accurate assessment of NF-κB pathway activity in cellular or tissue samples. Studies utilizing this antibody contribute to understanding how dysregulated NF-κB signaling drives pathological processes, aiding in therapeutic development targeting this pathway.