Rabbit Polyclonal Elk1(Phospho-Thr417) Antibody

The Elk1 (Phospho-Thr417) antibody is designed to specifically detect Elk1 protein when phosphorylated at threonine 417 (Thr417), a post-translational modification critical for its transcriptional activity. Elk1. a member of the ETS domain transcription factor family, acts as a downstream effector of the mitogen-activated protein kinase (MAPK) signaling pathway. It regulates the expression of immediate early genes (e.g., c-Fos) by binding to serum response elements (SREs) in promoter regions. Phosphorylation at Thr417. located within its transcriptional activation domain, enhances Elk1’s ability to recruit coactivators like the Mediator complex, thereby promoting target gene transcription. This modification typically occurs in response to extracellular stimuli, such as growth factors or stress signals, which activate upstream kinases like ERK1/2 or JNK. The Elk1 (Phospho-Thr417) antibody is widely used in studies investigating MAPK/ERK signaling dynamics, neuronal plasticity, cancer progression, and cellular responses to environmental cues. Its specificity enables researchers to assess Elk1 activation status in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP), providing insights into context-dependent signaling and gene regulation mechanisms. Validation often includes testing in knockout models or phosphatase-treated samples to confirm phosphorylation-dependent recognition.