Rabbit Polyclonal Myc(Phospho-Thr358) Antibody

The Myc(Phospho-Thr358) antibody is a specialized tool used to detect the phosphorylated form of the Myc protein at threonine residue 358. Myc, a proto-oncogene family (including c-Myc, N-Myc, and L-Myc), encodes transcription factors critical for regulating cell proliferation, differentiation, metabolism, and apoptosis. Post-translational modifications, such as phosphorylation, tightly control Myc protein stability, DNA-binding capacity, and transcriptional activity. Phosphorylation at Thr358. located in the Myc homology box 1 (MB1) domain of c-Myc, is implicated in modulating protein-protein interactions and downstream signaling. This site-specific phosphorylation may influence Myc’s oncogenic potential, as dysregulated Myc activity is linked to numerous cancers. The Myc(Phospho-Thr358) antibody enables researchers to study the activation status and regulatory mechanisms of Myc in cellular pathways. It is commonly used in techniques like Western blotting, immunoprecipitation, or immunofluorescence to assess phosphorylation dynamics in response to stimuli such as growth factors, stress, or pharmacological inhibitors. Understanding Myc phosphorylation at Thr358 provides insights into cancer biology, therapeutic targeting, and the interplay between oncogenic signaling networks and cell fate decisions. Validation of antibody specificity using knockout controls or phosphatase treatment is recommended to ensure accurate detection.