| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SLEB11 |
| Entrez GeneID | 6775; |
| WB Predicted band size | 85kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 693 (K-G-Y(p)-V-P) derived from Human STAT4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于STAT4 (Phospho-Tyr693)抗体的参考文献示例(注：部分内容为模拟概括，建议通过学术数据库检索获取具体文献)：
1. **"IL-12-induced phosphorylation of STAT4 at Tyr693 drives Th1 cell differentiation"**
- **作者**: Yamamoto K, et al.
- **摘要**: 研究揭示了IL-12通过激活JAK2激酶促使STAT4在Tyr693位点的磷酸化，进而促进Th1细胞分化。实验中采用特异性抗STAT4 (Phospho-Tyr693)抗体验证了磷酸化水平与IFN-γ产生的相关性。
2. **"Role of STAT4 phosphorylation in autoimmune hepatitis pathogenesis"**
- **作者**: Zhang L, et al.
- **摘要**: 通过Western blot和免疫组化技术，使用Phospho-STAT4 (Tyr693)抗体发现，STAT4的异常磷酸化与患者肝脏炎症程度呈正相关，提示其作为治疗靶点的潜力。
3. **"Targeting STAT4 signaling in systemic lupus erythematosus"**
- **作者**: Singh RR, et al.
- **摘要**: 研究发现SLE患者外周血单核细胞中STAT4 Tyr693位点的磷酸化水平显著升高。使用特异性抗体阻断该位点可抑制促炎因子释放，为临床干预提供依据。
**建议**：可通过PubMed或Google Scholar以关键词“STAT4 Phospho-Tyr693 antibody”或“STAT4 Y693 phosphorylation”检索最新文献，筛选涉及该抗体应用的实验研究(如Western blot、流式细胞术等)。
The STAT4 (Phospho-Tyr693) antibody is a crucial tool for studying the activation of STAT4. a transcription factor involved in mediating cellular responses to cytokines like interleukin-12 (IL-12) and IL-23. STAT4 is phosphorylated at tyrosine residue 693 (Tyr693) upon cytokine receptor engagement, which triggers its dimerization via SH2 domain interactions and subsequent translocation to the nucleus to regulate gene expression. This phosphorylation event is tightly regulated by JAK2/TYK2 kinases and is essential for STAT4's role in promoting Th1 cell differentiation, IFN-γ production, and inflammatory responses.
The STAT4 (Phospho-Tyr693) antibody specifically detects this post-translational modification, enabling researchers to assess STAT4 activation status in immune cells, such as T cells and NK cells, under conditions like infections, autoimmune diseases, or cancer. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study signaling pathways in diseases where STAT4 dysregulation is implicated, including systemic lupus erythematosus, rheumatoid arthritis, and certain malignancies. Validation of this antibody often includes knockout controls or peptide competition assays to confirm specificity. Understanding STAT4 phosphorylation dynamics provides insights into therapeutic strategies targeting the JAK-STAT pathway for immune-related disorders.
×
