| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NR3C3; PGR; PRGR |
| Entrez GeneID | 5241; |
| WB Predicted band size | 99kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 190 (G-L-S(p)-P-A) derived from Human Progesterone Receptor. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Progesterone Receptor (Phospho-Ser190) 抗体的3篇代表性文献摘要：
1. **"Phosphorylation of human progesterone receptor at serine-190 regulates ligand-independent receptor activity"**
- **作者**: Daniel A.R. et al. (2009)
- **摘要**: 研究揭示了PR Ser190位点的磷酸化在缺乏孕酮的情况下激活受体转录活性的机制，通过特异性抗体验证了该修饰在乳腺癌细胞中的功能，证明其与激素治疗耐药性相关。
2. **"MAPK-dependent phosphorylation of progesterone receptor Ser190: a key switch in receptor signaling"**
- **作者**: Qiu Y. et al. (2003)
- **摘要**: 利用Phospho-Ser190特异性抗体，发现MAPK信号通路通过磷酸化PR Ser190调控受体核转位和靶基因表达，提示该位点在细胞增殖中的作用。
3. **"CDK2-mediated phosphorylation of progesterone receptor Ser190 links cell cycle progression to transcriptional regulation"**
- **作者**: Hagan C.R., Asim M. (2015)
- **摘要**: 通过免疫沉淀和Western blot(使用Phospho-Ser190抗体)，证实CDK2在细胞周期G1/S期磷酸化PR Ser190.促进其与共激活因子的结合，影响乳腺癌进展。
4. **"Progesterone receptor phosphorylation in breast cancer: diagnostic and therapeutic implications"**
- **作者**: Smith C.L. et al. (2012)
- **摘要**: 研究利用Phospho-Ser190抗体分析乳腺癌组织样本，发现该位点磷酸化水平与肿瘤侵袭性相关，为靶向治疗提供潜在标志物。
(注：以上文献为模拟示例，实际引用需核实具体来源。)
The progesterone receptor (PR), a member of the nuclear receptor superfamily, mediates progesterone signaling critical for reproductive functions, embryo implantation, and mammary gland development. PR exists as two isoforms (PRA and PRB) generated from a single gene, with phosphorylation playing a key regulatory role in its activity, stability, and subcellular localization. The Phospho-Ser190 epitope specifically marks PR phosphorylation at serine 190. a post-translational modification associated with ligand-dependent activation and receptor conformational changes. This phosphorylation event is linked to PR dimerization, DNA binding, and interaction with co-regulators, influencing target gene transcription.
Antibodies targeting PR(Phospho-Ser190) are essential tools for studying PR activation dynamics in hormone-responsive tissues and diseases like breast cancer, where PR status guides endocrine therapy decisions. They enable detection of activated PR forms in techniques such as Western blotting, immunofluorescence, or immunohistochemistry. Researchers use these antibodies to explore PR signaling crosstalk with growth factor pathways or the impact of kinase inhibitors on receptor function. Specificity validation via phosphatase treatment or peptide competition is recommended, as phosphorylation-dependent antibodies may cross-react with unrelated epitopes. Such studies advance understanding of progesterone's role in physiology, cancer progression, and therapeutic resistance.
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