| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAXI; PXN; |
| Entrez GeneID | 5829; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Paxillin(Phospho-Tyr118)抗体相关的文献摘要概括：
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1. **文献名称**: *"Paxillin phosphorylation at Tyr118 regulates cell migration through differential association with Crk and Nck adaptors"*
**作者**: Brown MC, Turner CE
**摘要**: 研究揭示了Paxillin Tyr118磷酸化在细胞迁移中的分子机制，发现该位点的磷酸化通过选择性结合Crk或Nck适配蛋白，调控肌动蛋白细胞骨架重组和迁移信号通路。
2. **文献名称**: *"Phosphorylation of paxillin at tyrosine 118 promotes focal adhesion disassembly in endothelial cells"*
**作者**: Zaidel-Bar R, Milo R, et al.
**摘要**: 通过内皮细胞模型，证明Tyr118磷酸化促进黏着斑解聚，并利用特异性抗体验证其与FAK激酶的相互作用，揭示了该位点在血管生成中的作用。
3. **文献名称**: *"Mechanical force-induced phosphorylation of paxillin at tyrosine 118 regulates traction stress dynamics"*
**作者**: Wang Y, Gilmore A, et al.
**摘要**: 结合力学实验与分子生物学手段，发现机械力通过激活Src激酶诱导Tyr118磷酸化，从而调控细胞收缩力分布，为癌症转移提供潜在机制解释。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核对最新研究。若需实验方法类文献，可关注使用该抗体进行WB/IF/IHC验证的原始研究。
The Paxillin (Phospho-Tyr118) antibody is a crucial tool for studying focal adhesion dynamics and cell signaling. Paxillin, a multifunctional adaptor protein, localizes to focal adhesions and plays a central role in integrating signals from integrins, growth factor receptors, and cytoskeletal components. Phosphorylation at tyrosine 118 (Y118) is a key regulatory modification triggered by FAK (focal adhesion kinase) and Src family kinases during integrin-mediated cell adhesion, migration, and mechanotransduction. This phosphorylation creates binding sites for SH2 domain-containing proteins, facilitating downstream signaling cascades involving Rac/Rho GTPases and cytoskeletal reorganization.
The Paxillin (Phospho-Tyr118) antibody specifically recognizes this post-translational modification, enabling researchers to monitor activation states of paxillin in various cellular processes. It has broad applications in Western blotting, immunofluorescence, and immunohistochemistry to investigate cell-matrix interactions, cancer metastasis, wound healing, and embryonic development. Studies using this antibody have revealed its significance in pathological conditions, including tumor invasion (enhanced Y118 phosphorylation correlates with metastatic potential) and cardiovascular remodeling. Validation typically includes testing in kinase-inhibitor-treated or FAK/Src-deficient cells to confirm phosphorylation dependency. Proper controls are essential due to potential cross-reactivity with other phosphotyrosine residues or proteins.
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