| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | MFI2 |
| Uniprot No | Q9R0R1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-708aa |
| 氨基酸序列 | MRLLSVTFWL LLSLRTVVCV MEVQWCTISD AEQQKCKDMS EAFQGAGIRP SLLCVQGNSA DHCVQLIKEQ KADAITLDGG AIYEAGKEHG LKPVVGEVYD QDIGTSYYAV AVVRRNSNVT INTLKGVKSC HTGINRTVGW NVPVGYLVES GHLSVMGCDV LKAVGDYFGG SCVPGTGETS HSESLCRLCR GDSSGHNVCD KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGNT LPSWGKSLMS EDFQLLCRDG SRADITEWRR CHLAKVPAHA VVVRGDMDGG LIFQLLNEGQ LLFSHEDSSF QMFSSKAYSQ KNLLFKDSTL ELVPIATQNY EAWLGQEYLQ AMKGLLCDPN RLPHYLRWCV LSAPEIQKCG DMAVAFSRQN LKPEIQCVSA ESPEHCMEQI QAGHTDAVTL RGEDIYRAGK VYGLVPAAGE LYAEEDRSNS YFVVAVARRD SSYSFTLDEL RGKRSCHPYL GSPAGWEVPI GSLIQRGFIR PKDCDVLTAV SQFFNASCVP VNNPKNYPSA LCALCVGDEK GRNKCVGSSQ ERYYGYSGAF RCLVEHAGDV AFVKHTTVFE NTNGHNPEPW ASHLRWQDYE LLCPNGARAE VDQFQACNLA QMPSHAVMVR PDTNIFTVYG LLDKAQDLFG DDHNKNGFQM FDSSKYHSQD LLFKDATVRA VPVREKTTYL DWLGPDYVVA LEGMLSQQ |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于MFI2(Melanotransferrin)相关研究的模拟参考文献示例。由于实际文献可能存在差异，建议通过学术数据库进一步验证：
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1. **文献名称**: "Recombinant human melanotransferrin: expression in Pichia pastoris and functional characterization"
**作者**: Smith A, et al.
**摘要**: 本研究利用毕赤酵母系统表达了重组人源MFI2蛋白，优化了纯化流程并验证了其与铁离子的结合能力。结果显示重组MFI2在体外可促进细胞铁摄取，提示其潜在治疗缺铁相关疾病的用途。
2. **文献名称**: "MFI2 overexpression in cancer cells: role of recombinant protein in tumor progression"
**作者**: Lee JH, et al.
**摘要**: 通过大肠杆菌表达系统制备重组MFI2蛋白，发现其能增强多种癌细胞系的迁移和侵袭能力。机制研究表明，MFI2通过调控基质金属蛋白酶(MMPs)促进肿瘤转移。
3. **文献名称**: "Development of a monoclonal antibody against recombinant MFI2 for diagnostic applications"
**作者**: García R, et al.
**摘要**: 研究成功在CHO细胞中表达了高纯度MFI2重组蛋白，并基于此开发了特异性单克隆抗体。该抗体在ELISA和免疫组化中表现出高灵敏度和特异性，可用于癌症患者的血清标志物检测。
4. **文献名称**: "Structural and functional analysis of melanotransferrin using recombinant protein technology"
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了重组MFI2蛋白的三维结构，揭示了其与转铁蛋白家族的结构差异。功能实验表明，MFI2可能通过非经典途径参与细胞铁稳态调控。
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**备注**：以上文献信息为模拟生成，实际引用时请通过PubMed、Web of Science等平台核实具体研究内容及作者信息。如需进一步协助定位真实文献，请提供更详细的关键词或研究背景。
**Background of MFI2 Recombinant Protein**
MFI2 (Melanotransferrin), also known as MTF or CD228. is a member of the transferrin family of iron-binding proteins. Initially identified in melanoma cells, it shares structural homology with serum transferrin and lactoferrin but exhibits distinct functional properties. Unlike serum transferrin, which circulates in blood to regulate systemic iron transport, MFI2 is predominantly membrane-bound via a glycosylphosphatidylinositol (GPI) anchor or exists in a soluble form, suggesting roles in localized cellular iron homeostasis. The protein consists of two homologous lobes capable of binding ferric ions, though its iron-binding affinity and release mechanisms differ from classical transferrins.
MFI2 is implicated in diverse physiological and pathological processes. It is highly expressed in certain cancers, including melanoma and glioblastoma, where it may contribute to tumor cell survival by modulating iron uptake or mitigating oxidative stress. Studies suggest MFI2 influences cell proliferation, migration, and angiogenesis, potentially through iron-independent pathways, such as interactions with integrins or proteases. Its overexpression in malignancies has sparked interest in MFI2 as a biomarker or therapeutic target.
Recombinant MFI2 protein is engineered for research and potential clinical applications. Produced via expression systems like mammalian cells or *E. coli*, the purified protein retains functional domains for iron binding and receptor interaction. Researchers utilize it to study MFI2's role in iron metabolism, cancer biology, and cellular signaling. In drug discovery, it serves as an antigen for antibody development or a tool to explore iron-chelation strategies. Despite progress, MFI2's precise mechanisms and therapeutic potential remain under investigation, highlighting its relevance in both basic and applied biomedical research.
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