Rabbit Polyclonal AndrogenReceptor(Phospho-Ser650) Antibody

The Androgen Receptor (AR), a member of the nuclear receptor superfamily, mediates testosterone and dihydrotestosterone signaling, regulating gene expression critical for development, reproduction, and disease progression (e.g., prostate cancer). Post-translational modifications, including phosphorylation, modulate AR activity, stability, and subcellular localization. The Phospho-Ser650 epitope specifically marks AR phosphorylation at serine 650. a residue located in the hinge region between the DNA-binding domain (DBD) and ligand-binding domain (LBD). This phosphorylation event is associated with ligand-dependent receptor activation and subsequent nuclear translocation. Studies suggest that Ser650 phosphorylation may influence AR transcriptional activity, protein-protein interactions, or response to anti-androgen therapies. Antibodies targeting AR(Phospho-Ser650) are widely used in Western blotting, immunohistochemistry, and immunofluorescence to investigate AR activation states, particularly in prostate cancer models. Elevated phosphorylation at this site has been observed in advanced prostate cancer and during androgen-ablation therapy resistance, implicating its role in disease progression. These antibodies help researchers dissect AR signaling dynamics, evaluate therapeutic responses, and identify mechanisms underlying castration-resistant prostate cancer. Validation typically includes testing in androgen-stimulated cell lines or tissues with known AR activation status.