| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC25M1; MPIP3; |
| Entrez GeneID | 995; |
| WB Predicted band size | 60 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDC25C(Phospho-Ser216)抗体的3篇参考文献(示例为模拟内容，实际文献需根据具体数据库查询)：
1. **文献名称**：*"Cell cycle regulation of CDC25C phosphorylation and subcellular localization"*
**作者**：Kumagai A, et al.
**摘要**：研究CDC25C在细胞周期中的调控机制，通过Phospho-Ser216特异性抗体证实DNA损伤后该位点磷酸化，导致CDC25C滞留于细胞质并抑制CDK1激活。
2. **文献名称**：*"Phosphorylation of CDC25C at Ser216 promotes 14-3-3 protein binding and G2/M arrest"*
**作者**：Peng CY, et al.
**摘要**：利用Phospho-Ser216抗体验证DNA损伤应答中14-3-3蛋白与CDC25C的结合，阐明该磷酸化事件在G2/M期检查点中的关键作用。
3. **文献名称**：*"Targeting CDC25C phosphorylation as a therapeutic strategy in cancer"*
**作者**：Peng Y, et al.
**摘要**：通过Western blot和免疫荧光实验(使用Phospho-Ser216抗体)，探讨抑制CDC25C磷酸化对癌细胞周期阻滞的影响及潜在治疗价值。
如需具体文献，建议通过PubMed或Sci-Hub输入关键词“CDC25C Ser216 phosphorylation antibody”进一步筛选。
The Cdc25C (Phospho-Ser216) antibody detects the phosphorylated form of Cdc25C at serine 216. a key regulatory site involved in cell cycle control. Cdc25C, a member of the Cdc25 phosphatase family, plays a critical role in the G2/M transition by dephosphorylating and activating cyclin-dependent kinase 1 (CDK1), driving mitotic entry. Phosphorylation at Ser216. mediated by checkpoint kinases like Chk1/Chk2 in response to DNA damage or replication stress, creates a binding site for 14-3-3 proteins. This interaction sequesters Cdc25C in the cytoplasm, preventing its nuclear localization and CDK1 activation, thereby inducing cell cycle arrest to allow DNA repair.
The Cdc25C (Phospho-Ser216) antibody is widely used in research to study DNA damage checkpoints, cell cycle regulation, and mechanisms underlying genomic stability. It is particularly valuable in assays such as Western blotting, immunofluorescence, or flow cytometry to monitor checkpoint activation in response to genotoxic agents (e.g., radiation, chemotherapeutics). Dysregulation of this phosphorylation event has been implicated in cancer progression and resistance to therapy, making it a potential biomarker for therapeutic targeting. Researchers often pair this antibody with total Cdc25C antibodies to assess the ratio of phosphorylated to unmodified protein, providing insights into pathway activity. Proper sample preparation, including phosphatase inhibition, is essential to preserve phosphorylation signals during experiments.
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