Rabbit Polyclonal p53(Phospho-Ser315) Antibody

The p53 protein, a critical tumor suppressor, plays a central role in regulating cell cycle arrest, DNA repair, and apoptosis in response to cellular stress. Post-translational modifications, including phosphorylation, tightly modulate its stability and activity. Phosphorylation at Ser315 (p53 phospho-Ser315) is associated with cell cycle progression and occurs during mitosis, mediated by kinases such as Aurora-A or CDK1/cyclin B. This modification promotes p53 interaction with the Pin1 prolyl isomerase, facilitating conformational changes that influence its transcriptional activity or degradation. Additionally, Ser315 phosphorylation may regulate p53 nuclear export or its binding to target gene promoters under specific conditions. Antibodies specific for p53 phosphorylated at Ser315 are essential tools for studying p53 dynamics during cell cycle checkpoints, stress responses, and cancer biology. They enable detection of this modified form in techniques like Western blotting, immunofluorescence, or immunohistochemistry, helping researchers explore how Ser315 phosphorylation impacts p53's tumor-suppressive functions or oncogenic mutant p53 gain-of-function activities in diseases like colorectal, breast, or ovarian cancers. These antibodies are particularly valuable for investigating DNA damage response pathways and therapeutic resistance mechanisms.