| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCP; AFCP |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与Akt2 (Phospho-Ser474)抗体相关的文献摘要信息：
---
1. **"Phosphorylation of Akt at Ser473 correlates with poor prognosis in breast cancer"**
- **作者**: S. Tokunaga et al.
- **摘要**: 研究乳腺癌中Akt2 Ser474位点的磷酸化水平与临床预后的关系，发现高磷酸化Akt2与肿瘤转移和化疗耐药性相关，使用Phospho-Ser474抗体进行免疫组化分析。
---
2. **"Regulation of insulin sensitivity by phosphorylation of Akt2 on Ser474 in adipose tissue"**
- **作者**: J.M. Hoehn et al.
- **摘要**: 探讨脂肪组织中Akt2 Ser474磷酸化对胰岛素信号通路的调控作用，通过Western blot结合特异性Phospho-Ser474抗体，证明该位点磷酸化缺失导致胰岛素抵抗和代谢紊乱。
---
3. **"Akt2 kinase suppresses gluconeogenesis by targeting FOXO1 in the liver"**
- **作者**: X. Li et al.
- **摘要**: 研究Akt2在肝脏糖异生中的作用，发现Ser474磷酸化是Akt2激活的关键步骤，通过磷酸化抗体验证其在糖尿病模型中的表达变化，并揭示其通过FOXO1调控糖代谢的机制。
---
4. **"Site-specific phosphorylation of Akt by PDK1 and mTORC2 controls cell survival"**
- **作者**: C.C. Thoreen et al.
- **摘要**: 揭示Akt2 Ser474位点磷酸化由mTORC2激酶调控，使用Phospho-Ser474特异性抗体在基因敲除细胞模型中验证该位点对细胞存活和增殖的影响。
---
以上文献均涉及Akt2 Ser474磷酸化功能研究，并明确提及使用特异性抗体的实验验证。
The Akt2 (Phospho-Ser474) antibody is a key tool for studying the activation status of Akt2. a serine/threonine kinase critical in regulating cellular processes like metabolism, survival, and proliferation. Akt2. a member of the protein kinase B (PKB)/Akt family, is activated via phosphorylation at specific residues. Ser474 (or Ser473 in some isoforms/species) lies in the hydrophobic motif of Akt2 and is phosphorylated by mTORC2. a step essential for full kinase activation. This phosphorylation event facilitates downstream signaling through effectors like GSK-3β and FoxO transcription factors, influencing insulin sensitivity, glucose uptake, and apoptosis. Dysregulation of Akt2 phosphorylation is linked to metabolic disorders (e.g., diabetes) and cancer.
The Akt2 (Phospho-Ser474) antibody specifically detects Akt2 when phosphorylated at Ser474. enabling researchers to assess its activation in response to stimuli such as insulin or growth factors. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study tissue-specific or disease-associated Akt2 signaling. Validation typically includes testing in knockout models or phosphatase-treated samples to confirm specificity against non-phosphorylated Akt2 and other Akt isoforms (Akt1/Akt3). This antibody is valuable in research on insulin resistance, tumor progression, and therapeutic targeting of the PI3K/Akt pathway.
×
